C9orf72-Derived Proline:Arginine Poly-Dipeptides Modulate Cytoskeleton and Mechanical Stress Response.

• 資料種別: 博士論文
• タイトル: C9orf72-Derived Proline:Arginine Poly-Dipeptides Modulate Cytoskeleton and Mechanical Stress Response.
• 著者・編者: Shiota, Tomo; Nagata, Riko; Kikuchi, Sotaro; Nanaura, Hitoki; Matsubayashi, Masaya; Nakanishi, Mari; Kobashigawa, Shinko; Isozumi, Noriyoshi; Kiriyama, Takao; Nagayama, Kazuaki; Sugie, Kazuma; Yamashiro, Yoshito; Mori, Eiichiro
• 著者標目: Shiota, Tomo; Nagata, Riko; Kikuchi, Sotaro; Nanaura, Hitoki; Matsubayashi, Masaya; Nakanishi, Mari; Kobashigawa, Shinko; Isozumi, Noriyoshi; Kiriyama, Takao; Nagayama, Kazuaki; Sugie, Kazuma; Yamashiro, Yoshito; Mori, Eiichiro
• 出版事項: Frontiers Media S.A.
• 出版年月日等: 2022-03-23; 10
• 出版年（W3CDTF）: 2022-03-23
• 並列タイトル等: C9orf72由来のプロリン : アルギニンポリペプチドは細胞骨格とメカニカルストレス応答を制御する
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2022-09-28
• 授与年月日（W3CDTF）: 2022-09-28
• 報告番号: 甲第846号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: C9orf72; PR poly-dipeptides; cytoskeleton; Focal Adhesion (FA); actin
• 対象利用者: 一般
• 一般注記: type:Thesis; Proline:arginine (PR) poly-dipeptides from the GGGGCC repeat expansion in C9orf72 have cytotoxicity and bind intermediate filaments (IFs). However, it remains unknown how PR poly-dipeptides affect cytoskeletal organization and focal adhesion (FA) formation. Here, we show that changes to the cytoskeleton and FA by PR poly-dipeptides result in the alteration of cell stiffness and mechanical stress response. PR poly-dipeptides increased the junctions and branches of the IF network and increased cell stiffness. They also changed the distribution of actin filaments and increased the size of FA and intracellular calcium concentration. PR poly-dipeptides or an inhibitor of IF organization prevented cell detachment. Furthermore, PR poly-dipeptides induced upregulation of mechanical stress response factors and led to a maladaptive response to cyclic stretch. These results suggest that the effects of PR poly-dipeptides on mechanical properties and mechanical stress response may serve as a pathogenesis of C9orf72-related neurodegeneration.; 博士（医学）・甲第846号・令和4年9月28日; Copyright © 2022 Shiota, Nagata, Kikuchi, Nanaura, Matsubayashi, Nakanishi,Kobashigawa, Isozumi, Kiriyama, Nagayama, Sugie, Yamashiro and Mori. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.; identifier:Frontiers in cell and developmental biology Vol.10 Article No.750829 (2022 Mar); identifier:2296634X; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4073; identifier:Frontiers in cell and developmental biology, 10: Article No.750829
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• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2023-01-30T13:49:34+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10564/4073
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp