Utilization of Natural Detergent Potassium Laurate for Decellularization in Lung Bioengineering 8

• 資料種別: 博士論文
• タイトル: Utilization of Natural Detergent Potassium Laurate for Decellularization in Lung Bioengineering
• 巻次・部編番号: 25 8
• 著者・編者: 小畑, 智裕
• 著者標目: 小畑, 智裕
• 出版事項: Mary Ann Liebert, Inc.
• 出版年月日等: 2022-09-07; 25 8
• 出版年（W3CDTF）: 2022-09-07
• 並列タイトル等: 肺再生の脱細胞化技術における天然洗浄剤ラウリン酸カリウムの有用性
• タイトル（掲載誌）: Tissue Engineering Part C: Methods
• 掲載ページ: 459-471
• ISSN（掲載誌）: 21524947
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2022-09-07
• 授与年月日（W3CDTF）: 2022-09-07
• 報告番号: 甲医歯薬第1463号
• 学位: 博士(医学)
• 本文の言語コード: eng
• 件名標目: decellularization; lung bioengineering; natural detergent; potassium laurate; lung transplantation
• 対象利用者: 一般
• 一般注記: Perfusion decellularization by detergents is used to harvest native organ scaffolds with delicate three-dimensional structures from the natural extracellular matrix (ECM). However, detergents often damage ECM proteins, disrupting the reseeding of cells during the bioengineering process. In this study, we analyzed the ability of the natural soap potassium laurate (PL), generated from a natural fatty acid, to act as a detergent for lung bioengineering. Harvested rat lungs were decellularized by PL or the conventional detergent sodium dodecyl sulfate (SDS). The results indicated that PL could remove cells and DNA. Moreover, PL-decellularized lungs showed obviously improved preservation of architecture, with elastin microfibrils, sulfated glycosaminoglycans, and ECM proteins, compared with that with SDS. Mesenteric implanted PL-decellularized scaffolds demonstrated significantly reduced inflammatory reactions compared with SDS-decellularized scaffolds. In vitro, rat lung microvascular epithelial cell (RLMVEC) proliferation and survival rates were increased by coculturing with minced PL-decellularized scaffolds but not minced SDS-decellularized scaffolds. After recellularization using RLMVECs and adipogenic stem/stromal cells, recellularized cells uniformly distributed in PL-decellularized scaffolds but not in SDS-decellularized scaffolds. After the transplantation of bioengineered lungs, lung congestion was obviously reduced with PL-decellularized bioengineered tissues. Overall, our findings suggest that PL is very well suited for organ bioengineering.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1463号 学位授与年月日:令和4年9月7日; Author: Tomohiro Obata, Tomoshi Tsuchiya, Sadanori Akita, Takayoshi Kawahara, Keitaro Matsumoto, Takuro Miyazaki, Hiroshi Masumoto, Eiji Kobayashi, Laura E. Niklason, and Takeshi Nagayasu; Citation: Tissue Engineering Part C: Methods, 25(8), pp. 459-471; 2019; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-09-07); 機関リポジトリ記載の権利情報: Copyright 2019, Mary Ann Liebert, Inc., publishers. This is the accepted version of the following article: Tissue Engineering Part C: Methods, 25(8), pp. 459-471; 2019, which has now been formally published in final form at Tissue Engineering Part C: Methods at https://doi.org/10.1089/ten.tec.2019.0016. This original submission version of the article may be used for non-commercial purposes in accordance with the Mary Ann Liebert, Inc., publishers' self-archiving terms and conditions.
• 国立国会図書館永続的識別子: info:ndljp/pid/12651196; https://dl.ndl.go.jp/pid/12651196
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2023-03-02T11:18:09+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 関連情報（URI）: https://doi.org/10.1089/ten.tec.2019.0016
• 掲載誌（URI）: http://hdl.handle.net/10069/00041874
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp