Utilization of Natural Detergent Potassium Laurate for Decellularization in Lung Bioengineering

• Material Type: 博士論文
• Title: Utilization of Natural Detergent Potassium Laurate for Decellularization in Lung Bioengineering
• Author/Editor: 小畑, 智裕
• Author Heading: 小畑, 智裕
• Publication, Distribution, etc.: Mary Ann Liebert, Inc.
• Publication Date: 2022-09-07
• Publication Date (W3CDTF): 2022-09-07
• Alternative Title: 肺再生の脱細胞化技術における天然洗浄剤ラウリン酸カリウムの有用性
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2022-09-07
• Date Granted (W3CDTF): 2022-09-07
• Dissertation Number: 甲医歯薬第1463号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: decellularization; lung bioengineering; natural detergent; potassium laurate; lung transplantation
• Target Audience: 一般
• Note (General): Perfusion decellularization by detergents is used to harvest native organ scaffolds with delicate three-dimensional structures from the natural extracellular matrix (ECM). However, detergents often damage ECM proteins, disrupting the reseeding of cells during the bioengineering process. In this study, we analyzed the ability of the natural soap potassium laurate (PL), generated from a natural fatty acid, to act as a detergent for lung bioengineering. Harvested rat lungs were decellularized by PL or the conventional detergent sodium dodecyl sulfate (SDS). The results indicated that PL could remove cells and DNA. Moreover, PL-decellularized lungs showed obviously improved preservation of architecture, with elastin microfibrils, sulfated glycosaminoglycans, and ECM proteins, compared with that with SDS. Mesenteric implanted PL-decellularized scaffolds demonstrated significantly reduced inflammatory reactions compared with SDS-decellularized scaffolds. In vitro, rat lung microvascular epithelial cell (RLMVEC) proliferation and survival rates were increased by coculturing with minced PL-decellularized scaffolds but not minced SDS-decellularized scaffolds. After recellularization using RLMVECs and adipogenic stem/stromal cells, recellularized cells uniformly distributed in PL-decellularized scaffolds but not in SDS-decellularized scaffolds. After the transplantation of bioengineered lungs, lung congestion was obviously reduced with PL-decellularized bioengineered tissues. Overall, our findings suggest that PL is very well suited for organ bioengineering.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1463号 学位授与年月日:令和4年9月7日; Author: Tomohiro Obata, Tomoshi Tsuchiya, Sadanori Akita, Takayoshi Kawahara, Keitaro Matsumoto, Takuro Miyazaki, Hiroshi Masumoto, Eiji Kobayashi, Laura E. Niklason, and Takeshi Nagayasu; Citation: Tissue Engineering Part C: Methods, 25(8), pp. 459-471; 2019; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-09-07); http://hdl.handle.net/10069/00041829
• Persistent ID (NDL): info:ndljp/pid/12651196; https://dl.ndl.go.jp/pid/12651196
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-03-02T11:18:09+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://hdl.handle.net/10069/00041874
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp