並列タイトル等堆積物に生息するメタン生成古細菌のメタゲノム解析に関する研究
一般注記This study identified methanogenic communities in estuary and wetland ecosystems based on 16S rRNA gene sequencing and molecular cloning of the functional mcrA gene. Well-known methanogenic archaea (mainly Methanomicrobiales) and uncultivated putative methanogenic archaea (Verstraetearchaeota and Methanofastidiosales) were detected in the estuarine sediments, indicating the CH₄ production potentials from hydrogenotrophic and H₂-dependent methylotrophic methanogenesis. For wetland peat soils, the “Candidatus Bathyarchaeota” was highly observed (up to 96% of the archaeal abundance) based on microbial gene quantification. Furthermore, partial fragments of the mcrA gene, belonging to the Bathyarchaeota, was detected based on the functional gene calling and metagenomic reconstruction. These results firstly demonstrated that Bathyarchaeota that possess the mcrA gene and its potential in methane production could be observed in a natural wetland ecosystem. The discovery of novel Mcr and Mcr-like genes provides a great discussion and interpretation of the origin and evolutionary history of the mcrA and Mcr complex.
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受理日（W3CDTF）2023-03-02T11:18:12+09:00
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