Diversity of methanogenic archaea in the natural subsurface sediments revealed by culture-independent genomic approaches

• Material Type: 博士論文
• Title: Diversity of methanogenic archaea in the natural subsurface sediments revealed by culture-independent genomic approaches
• Author/Editor: Prasitwuttisak, Wipoo
• Author Heading: Prasitwuttisak, Wipoo
• Publication Date: 2022-09-26
• Publication Date (W3CDTF): 2022-09-26
• Alternative Title: 堆積物に生息するメタン生成古細菌のメタゲノム解析に関する研究
• Degree grantor/type: 北九州市立大学
• Date Granted: 2022-09-26
• Date Granted (W3CDTF): 2022-09-26
• Dissertation Number: 甲第199号
• Degree Type: 博士(工学)
• Text Language Code: eng
• Subject Heading: estuary; wetland; methanogenesis; methanogenic archaea; mcrA; Candidatus Bathyarchaeota
• Target Audience: 一般
• Note (General): This study identified methanogenic communities in estuary and wetland ecosystems based on 16S rRNA gene sequencing and molecular cloning of the functional mcrA gene. Well-known methanogenic archaea (mainly Methanomicrobiales) and uncultivated putative methanogenic archaea (Verstraetearchaeota and Methanofastidiosales) were detected in the estuarine sediments, indicating the CH₄ production potentials from hydrogenotrophic and H₂-dependent methylotrophic methanogenesis. For wetland peat soils, the “Candidatus Bathyarchaeota” was highly observed (up to 96% of the archaeal abundance) based on microbial gene quantification. Furthermore, partial fragments of the mcrA gene, belonging to the Bathyarchaeota, was detected based on the functional gene calling and metagenomic reconstruction. These results firstly demonstrated that Bathyarchaeota that possess the mcrA gene and its potential in methane production could be observed in a natural wetland ecosystem. The discovery of novel Mcr and Mcr-like genes provides a great discussion and interpretation of the origin and evolutionary history of the mcrA and Mcr complex.
• Persistent ID (NDL): info:ndljp/pid/12651854; https://dl.ndl.go.jp/pid/12651854
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-03-02T11:18:12+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): https://kitakyu.repo.nii.ac.jp/records/1040
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-09-07 再収集