Anti-CENP-C Antibody-Based Immunofluorescence Dicentric Assay: Radiation Dose-Response, Validation Studies, and Radiation Dose-Dependency on Sister Centromere Fluorescence 1

• 資料種別: 博士論文
• タイトル: Anti-CENP-C Antibody-Based Immunofluorescence Dicentric Assay: Radiation Dose-Response, Validation Studies, and Radiation Dose-Dependency on Sister Centromere Fluorescence
• 巻次・部編番号: 199 1
• 著者・編者: 氏家, 里紗
• 著者標目: 氏家, 里紗
• 出版事項: Radiation Research Society
• 出版年月日等: 2023-03-20; 199 1
• 出版年（W3CDTF）: 2023-03-20
• 並列タイトル等: 抗CENP-C抗体による蛍光免疫二動原体染色体解析法：放射線量依存性、検証評価、姉妹セントロメア蛍光強度の放射線量応答性
• タイトル（掲載誌）: Radiation Research
• 掲載ページ: 74-82
• ISSN（掲載誌）: 00337587
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2023-03-20
• 授与年月日（W3CDTF）: 2023-03-20
• 報告番号: 甲医歯薬第1525号
• 学位: 博士(医学)
• 本文の言語コード: eng
• 対象利用者: 一般
• 一般注記: Dicentric chromosome assay (DCA) is the most accepted cytological technique for the purpose of biological dosimetry in radiological and nuclear accidents, however, it is not always easy to evaluate dicentric chromosomes because of the technical difficulty in identifying dicentric chromosomes on Giemsa-stained metaphase chromosome samples. Here, we applied an antibody recognizing centromere protein (CENP) C, CENP-C, whose antigenicity is resistant to the fixation with Carnoy's solution. Normal human diploid cells were irradiated with various doses of 137Cs γ rays at 1 Gy/ min, treated with hypotonic solution, fixed with Carnoy's fixative, and metaphase chromosome spreads were stained with anti-CENP-C antibody. Dose-dependent induction of dicentric chromosomes was confirmed between 1 and 10 Gy of γ rays, and the results were compatible with those obtained by the conventional Giemsa-stained chromosome samples. The CENP-C assay also uncovered the difference in the fluorescence from the sister centromeres on the same chromosome, which was more pronounced after radiation exposure. Although the underlying mechanism is still to be determined, the result suggests a novel effect of radiation on centromeres. The innovative protocol for CENP-C-based DCA, which enables ideal visualization of centromeres, is simple, effective and reliable. It does not require skilled examiners, so that it may be an alternative method, avoiding uneasiness of the current DCA using Giemsa-stained metaphase chromosome samples.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1525号 学位授与年月日:令和5年3月20日; Author: Ujiie Risa, Kawamura Kasumi, Yamashita Shunichi, Mitsutake Norisato, Suzuki Keiji; Citation: Radiation Research, 199(1), pp.74-82; 2023; identifier:Nagasaki University (長崎大学), 博士(医学) (2023-03-20); 機関リポジトリ記載の権利情報: ⓒ 2023 by Radiation Research Society. All rights of reproduction in any form reserved.
• 国立国会図書館永続的識別子: info:ndljp/pid/12864103; https://dl.ndl.go.jp/pid/12864103
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2023-05-05T22:12:35+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 関連情報（URI）: https://doi.org/10.1667/RADE-22-00050.1
• 掲載誌（URI）: http://hdl.handle.net/10069/00042358
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp