Anti-CENP-C Antibody-Based Immunofluorescence Dicentric Assay: Radiation Dose-Response, Validation Studies, and Radiation Dose-Dependency on Sister Centromere Fluorescence

• Material Type: 博士論文
• Title: Anti-CENP-C Antibody-Based Immunofluorescence Dicentric Assay: Radiation Dose-Response, Validation Studies, and Radiation Dose-Dependency on Sister Centromere Fluorescence
• Author/Editor: 氏家, 里紗
• Author Heading: 氏家, 里紗
• Publication, Distribution, etc.: Radiation Research Society
• Publication Date: 2023-03-20
• Publication Date (W3CDTF): 2023-03-20
• Alternative Title: 抗CENP-C抗体による蛍光免疫二動原体染色体解析法：放射線量依存性、検証評価、姉妹セントロメア蛍光強度の放射線量応答性
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2023-03-20
• Date Granted (W3CDTF): 2023-03-20
• Dissertation Number: 甲医歯薬第1525号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Target Audience: 一般
• Note (General): Dicentric chromosome assay (DCA) is the most accepted cytological technique for the purpose of biological dosimetry in radiological and nuclear accidents, however, it is not always easy to evaluate dicentric chromosomes because of the technical difficulty in identifying dicentric chromosomes on Giemsa-stained metaphase chromosome samples. Here, we applied an antibody recognizing centromere protein (CENP) C, CENP-C, whose antigenicity is resistant to the fixation with Carnoy's solution. Normal human diploid cells were irradiated with various doses of 137Cs γ rays at 1 Gy/ min, treated with hypotonic solution, fixed with Carnoy's fixative, and metaphase chromosome spreads were stained with anti-CENP-C antibody. Dose-dependent induction of dicentric chromosomes was confirmed between 1 and 10 Gy of γ rays, and the results were compatible with those obtained by the conventional Giemsa-stained chromosome samples. The CENP-C assay also uncovered the difference in the fluorescence from the sister centromeres on the same chromosome, which was more pronounced after radiation exposure. Although the underlying mechanism is still to be determined, the result suggests a novel effect of radiation on centromeres. The innovative protocol for CENP-C-based DCA, which enables ideal visualization of centromeres, is simple, effective and reliable. It does not require skilled examiners, so that it may be an alternative method, avoiding uneasiness of the current DCA using Giemsa-stained metaphase chromosome samples.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1525号 学位授与年月日:令和5年3月20日; Author: Ujiie Risa, Kawamura Kasumi, Yamashita Shunichi, Mitsutake Norisato, Suzuki Keiji; Citation: Radiation Research, 199(1), pp.74-82; 2023; identifier:Nagasaki University (長崎大学), 博士(医学) (2023-03-20); http://hdl.handle.net/10069/00042266
• Persistent ID (NDL): info:ndljp/pid/12864103; https://dl.ndl.go.jp/pid/12864103
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-05-05T22:12:35+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10069/00042358
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp