Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage

• 資料種別: 博士論文
• タイトル: Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage
• 著者・編者: Kiyokawa, Yuki
• 著者標目: Kiyokawa, Yuki
• 出版年月日等: 2023-03-23
• 出版年（W3CDTF）: 2023-03-23
• 並列タイトル等: 薬剤誘導を行ったナイーブ型iPS細胞は、プライム型iPS細胞に比較して、膵β細胞系への分化能に優れている
• 授与機関名: 新潟大学
• 授与年月日: 2023-03-23
• 授与年月日（W3CDTF）: 2023-03-23
• 報告番号: 甲第5146号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 著者別名: 清川, 裕貴
• 件名標目: induced pluripotent stem cells; pancreatic β-cells; pancreas; naïve stem cells; drug induction; differentiation; epiblast stem cells; insulin-producing cells; intrapancreatic parenchymal cell transplantation (IPPCT); teratoma formation assay
• 対象利用者: 一般
• 一般注記: Pluripotent stem cells are classified as naïve and primed cells, based on their in vitro growth characteristics and potential to differentiate into various types of cells. Human-induced pluripotent stem cells (iPSCs, also known as epiblast stem cells [EpiSCs]) have limited capacity to differentiate and are slightly more differentiated than naïve stem cells (NSCs). Although there are several in vitro protocols that allow iPSCs to differentiate into pancreatic lineage, data concerning generation of β-cells from these iPSCs are limited. Based on the pluripotentiality of NSCs, it was hypothesized that NSCs can differentiate into pancreatic β-cells when placed under an appropriate differentiation induction condition. We examined whether NSCs can be efficiently induced to form potentially pancreatic β-cells after being subjected to an in vitro protocol. Several colonies resembling in vitro-produced β-cell foci, with β-cell-specific marker expression, were observed when NSC-derived embryoid bodies (EBs) were induced to differentiate into β-cell lineage. Conversely, EpiSC-derived EBs failed to form such foci in vitro. Intrapancreatic grafting of the in vitro-formed β-cell foci into nude mice (BALB/c-nu/nu) generated a cell mass containing insulin-producing cells (IPCs), without noticeable tumorigenesis. These NSCs can be used as a promising resource for curing type 1 diabetes.; Journal of Climinal Medicine. 2020, 9(9), 2838.; 新大院博(歯)第523号
• 国立国会図書館永続的識別子: info:ndljp/pid/12985122; https://dl.ndl.go.jp/pid/12985122
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2023-09-01T22:11:30+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10191/0002001084; https://niigata-u.repo.nii.ac.jp/records/2001084
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp