Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage

• Material Type: 博士論文
• Title: Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage
• Author/Editor: Kiyokawa, Yuki
• Author Heading: Kiyokawa, Yuki
• Publication Date: 2023-03-23
• Publication Date (W3CDTF): 2023-03-23
• Alternative Title: 薬剤誘導を行ったナイーブ型iPS細胞は、プライム型iPS細胞に比較して、膵β細胞系への分化能に優れている
• Degree grantor/type: 新潟大学
• Date Granted: 2023-03-23
• Date Granted (W3CDTF): 2023-03-23
• Dissertation Number: 甲第5146号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Alias of Author: 清川, 裕貴
• Subject Heading: induced pluripotent stem cells; pancreatic β-cells; pancreas; naïve stem cells; drug induction; differentiation; epiblast stem cells; insulin-producing cells; intrapancreatic parenchymal cell transplantation (IPPCT); teratoma formation assay
• Target Audience: 一般
• Note (General): Pluripotent stem cells are classified as naïve and primed cells, based on their in vitro growth characteristics and potential to differentiate into various types of cells. Human-induced pluripotent stem cells (iPSCs, also known as epiblast stem cells [EpiSCs]) have limited capacity to differentiate and are slightly more differentiated than naïve stem cells (NSCs). Although there are several in vitro protocols that allow iPSCs to differentiate into pancreatic lineage, data concerning generation of β-cells from these iPSCs are limited. Based on the pluripotentiality of NSCs, it was hypothesized that NSCs can differentiate into pancreatic β-cells when placed under an appropriate differentiation induction condition. We examined whether NSCs can be efficiently induced to form potentially pancreatic β-cells after being subjected to an in vitro protocol. Several colonies resembling in vitro-produced β-cell foci, with β-cell-specific marker expression, were observed when NSC-derived embryoid bodies (EBs) were induced to differentiate into β-cell lineage. Conversely, EpiSC-derived EBs failed to form such foci in vitro. Intrapancreatic grafting of the in vitro-formed β-cell foci into nude mice (BALB/c-nu/nu) generated a cell mass containing insulin-producing cells (IPCs), without noticeable tumorigenesis. These NSCs can be used as a promising resource for curing type 1 diabetes.; Journal of Climinal Medicine. 2020, 9(9), 2838.; 新大院博(歯)第523号
• Persistent ID (NDL): info:ndljp/pid/12985122; https://dl.ndl.go.jp/pid/12985122
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-09-01T22:11:30+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://hdl.handle.net/10191/0002001084; https://niigata-u.repo.nii.ac.jp/records/2001084
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp