Determination of AGEs in mice femur by LC-MS/MS

• 資料種別: 記事
• タイトル: Determination of AGEs in mice femur by LC-MS/MS
• 著者・編者: Yoshitaka Hiraoka; Hikari Sugawa; Ryoji Nagai
• 出版事項: 日本 : 糖化ストレス研究会
• 出版年月日等: 2023-03-31
• 出版年（W3CDTF）: 2023-03-31
• タイトル（掲載誌）: Glycative stress research
• 巻号年月日等（掲載誌）: 10(1)
• 掲載巻: 10(1)
• ISSN（掲載誌）: 2188-3610
• ISSN-L（掲載誌）: 2188-3610
• 本文の言語コード: eng
• DOI: 10.24659/gsr.10.1_1; https://doi.org/10.24659/gsr.10.1_1
• 国立国会図書館永続的識別子: info:ndljp/pid/13120793; https://dl.ndl.go.jp/pid/13120793
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• 収集根拠: オンライン資料収集制度
• 受理日（W3CDTF）: 2023-12-08T11:19:22+09:00
• 保存日（W3CDTF）: 2023-05-20
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: https://www.toukastress.jp/webj/
• 掲載誌（国立国会図書館永続的識別子）: info:ndljp/pid/13120792; https://dl.ndl.go.jp/pid/13120792
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 所蔵機関: 国立国会図書館
• 請求記号: Z63-D541
• 関連情報（URI）: https://dl.ndl.go.jp/info:ndljp/pid/13120793
• 関連情報（国立国会図書館永続的識別子）: info:ndljp/pid/13120793; https://dl.ndl.go.jp/info:ndljp/pid/13120793
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 033430106; http://id.ndl.go.jp/bib/033430106
• 整理区分コード: 632

• 要約等: In recent years, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been used in the research on advanced glycation end-products (AGEs), enabling the measurement of several types of AGEs. However, there still remain many unknowns regarding how AGE accumulate in tissues during aging and age-related diseases, and the relationship between each biological tissue and AGE accumulation. We have established a method for the determination of AGEs in various tissues using mice as an animal model and have quantified AGEs in various soft tissues, turning out to be difficult to quantify AGEs in bone tissue, which is a hard tissue, unlike soft tissue processed easily. In this study, we attempted to quantify AGEs in mouse femur using LC-MS/MS. As a result, <i>N^ε</i>-(carboxymethyl)lysine (CML) and <i>N^δ</i>-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1) were clearly detected in mouse femur, and the amount of CML in femur was significantly higher than that in the crystalline lens. These results confirm that AGE content is high in tissues such as bone, where metabolism and inflammatory reactions occur actively through the action of osteoblasts and osteoclasts. The fact that AGEs in hard tissues as well as soft tissues of mice can now be quantified using LC-MS/MS is expected to make it more effortless to study AGEs in rodents in the future.
• DOI: 10.24659/gsr.10.1_1
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: In recent years, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been used in the research on advanced glycation end-products (AGEs), enabling the measurement of several types of AGEs. However, there still remain many unknowns regarding how AGE accumulate in tissues during aging and age-related diseases, and the relationship between each biological tissue and AGE accumulation. We have established a method for the determination of AGEs in various tissues using mice as an animal model and have quantified AGEs in various soft tissues, turning out to be difficult to quantify AGEs in bone tissue, which is a hard tissue, unlike soft tissue processed easily. In this study, we attempted to quantify AGEs in mouse femur using LC-MS/MS. As a result, <i>N^ε</i>-(carboxymethyl)lysine (CML) and <i>N^δ</i>-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1) were clearly detected in mouse femur, and the amount of CML in femur was significantly higher than that in the crystalline lens. These results confirm that AGE content is high in tissues such as bone, where metabolism and inflammatory reactions occur actively through the action of osteoblasts and osteoclasts. The fact that AGEs in hard tissues as well as soft tissues of mice can now be quantified using LC-MS/MS is expected to make it more effortless to study AGEs in rodents in the future.
• DOI: 10.24659/gsr.10.1_1; https://doi.org/10.24659/gsr.10.1_1
• 関連情報（URI）: http://id.ndl.go.jp/bib/033430106; https://ndlsearch.ndl.go.jp/books/R000000004-I033430106; https://dl.ndl.go.jp/pid/13120793; http://id.ndl.go.jp/digimeta/13120793; https://search.jamas.or.jp/link/ui/2024180404
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 033430106; http://id.ndl.go.jp/bib/033430106; 13120793; http://id.ndl.go.jp/bib/13120793