Determination of AGEs in mice femur by LC-MS/MS
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DOI[10.24659/gsr.10.1_1]to the data of the same series
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- Material Type
- 記事
- Author/Editor
- Yoshitaka HiraokaHikari SugawaRyoji Nagai
- Publication, Distribution, etc.
- Publication Date
- 2023-03-31
- Publication Date (W3CDTF)
- 2023-03-31
- Periodical title
- Glycative stress research
- No. or year of volume/issue
- 10(1)
- Volume
- 10(1)
- ISSN (Periodical Title)
- 2188-3610
- ISSN-L (Periodical Title)
- 2188-3610
- Text Language Code
- eng
- DOI
- 10.24659/gsr.10.1_1
- Persistent ID (NDL)
- info:ndljp/pid/13120793
- Collection
- Collection (Materials For Handicapped People:1)
- Collection (particular)
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他
- Acquisition Basis
- オンライン資料収集制度
- Date Accepted (W3CDTF)
- 2023-12-08T11:19:22+09:00
- Date Captured (W3CDTF)
- 2023-05-20
- Format (IMT)
- application/pdf
- Access Restrictions
- 国立国会図書館内限定公開
- Service for the Digitized Contents Transmission Service
- 図書館・個人送信対象外
- Availability of remote photoduplication service
- 可
- Periodical Title (URI)
- Periodical Title (Persistent ID (NDL))
- info:ndljp/pid/13120792
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館デジタルコレクション
- Collection (particular)
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他
- Access Restrictions
- 国立国会図書館内限定公開
- Service for the Digitized Contents Transmission Service
- 図書館・個人送信対象外
- Availability of remote photoduplication service
- 可
- Holding library
- 国立国会図書館
- Call No.
- Z63-D541
- Related Material (URI)
- Related Material (Persistent ID (NDL))
- info:ndljp/pid/13120793
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館雑誌記事索引
- Bibliographic ID (NDL)
- 033430106
- Bibliographic Record Category (NDL)
- 632
- Summary, etc.
- In recent years, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been used in the research on advanced glycation end-products (AGEs), enabling the measurement of several types of AGEs. However, there still remain many unknowns regarding how AGE accumulate in tissues during aging and age-related diseases, and the relationship between each biological tissue and AGE accumulation. We have established a method for the determination of AGEs in various tissues using mice as an animal model and have quantified AGEs in various soft tissues, turning out to be difficult to quantify AGEs in bone tissue, which is a hard tissue, unlike soft tissue processed easily. In this study, we attempted to quantify AGEs in mouse femur using LC-MS/MS. As a result, <i>N<sup>ε</sup></i>-(carboxymethyl)lysine (CML) and <i>N<sup>δ</sup></i>-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1) were clearly detected in mouse femur, and the amount of CML in femur was significantly higher than that in the crystalline lens. These results confirm that AGE content is high in tissues such as bone, where metabolism and inflammatory reactions occur actively through the action of osteoblasts and osteoclasts. The fact that AGEs in hard tissues as well as soft tissues of mice can now be quantified using LC-MS/MS is expected to make it more effortless to study AGEs in rodents in the future.
- DOI
- 10.24659/gsr.10.1_1
- Access Restrictions
- インターネット公開
- Data Provider (Database)
- 科学技術振興機構 : J-STAGE
- Summary, etc.
- In recent years, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been used in the research on advanced glycation end-products (AGEs), enabling the measurement of several types of AGEs. However, there still remain many unknowns regarding how AGE accumulate in tissues during aging and age-related diseases, and the relationship between each biological tissue and AGE accumulation. We have established a method for the determination of AGEs in various tissues using mice as an animal model and have quantified AGEs in various soft tissues, turning out to be difficult to quantify AGEs in bone tissue, which is a hard tissue, unlike soft tissue processed easily. In this study, we attempted to quantify AGEs in mouse femur using LC-MS/MS. As a result, <i>N<sup>ε</sup></i>-(carboxymethyl)lysine (CML) and <i>N<sup>δ</sup></i>-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1) were clearly detected in mouse femur, and the amount of CML in femur was significantly higher than that in the crystalline lens. These results confirm that AGE content is high in tissues such as bone, where metabolism and inflammatory reactions occur actively through the action of osteoblasts and osteoclasts. The fact that AGEs in hard tissues as well as soft tissues of mice can now be quantified using LC-MS/MS is expected to make it more effortless to study AGEs in rodents in the future.
- DOI
- 10.24659/gsr.10.1_1
- Related Material (URI)
- Data Provider (Database)
- 国立情報学研究所 : CiNii Research
- Original Data Provider (Database)
- Japan Link Center雑誌記事索引データベース雑誌記事索引データベース
- Bibliographic ID (NDL)
- 03343010613120793