がん細胞におけるミトコンドリア由来活性酸素を介した3-Methyl pyruvateによる放射線感受性の増強

• 資料種別: 博士論文
• タイトル: がん細胞におけるミトコンドリア由来活性酸素を介した3-Methyl pyruvateによる放射線感受性の増強
• 著者・編者: 西田, 直哉
• 著者標目: 西田, 直哉
• 出版事項: Hokkaido University
• 出版年月日等: 2014-03-25
• 出版年（W3CDTF）: 2014-03-25
• 並列タイトル等: 3-Methyl pyruvate enhances the radiosensitivity through increase of mitochondria derivedreactive oxygen species in tumor cell lines
• 寄与者: 稲波, 修; 石塚, 真由美; 佐藤, 耕太; 山盛, 徹
• 授与機関名: 北海道大学
• 授与年月日: 2014-03-25
• 授与年月日（W3CDTF）: 2014-03-25
• 報告番号: 甲第11283号
• 学位: 博士(獣医学)
• 博論授与番号: 甲第11283号
• 本文の言語コード: jpn
• 著者別名: Nishida, Naoya
• NDC: 649
• 対象利用者: 一般
• 一般注記: Considerable interest has recently been focused on the special characteristics of cancer metabolism, and several drugs designed to modulate cancer metabolism have been tested as potential anticancer agents. To date, however, very few studies have been conducted to investigate the combined effects of anticancer drugs and radiotherapy. In this study, to evaluate the role of mitochondria-derived reactive oxygen species (ROS) in the radiation-induced cell death of tumor cells, we have examined the effect of 3-methyl pyruvate (MP). MP is a membrane-permeable pyruvate derivative that is capable of activating mitochondrial energy metabolism, on human lung carcinoma A549 cells and murine squamous carcinoma SCCVII cells. Pretreatment with MP significantly enhanced radiation-induced cell death in both cell lines, and also led to increases in themitochondrial membrane potential, intracellular adenosine triphosphate content, and mitochondria-derived ROS production following the exposure of the cells to X-rays. In A549 cells, MP-induced radiosensitization was completely abolished by vitamin C. In contrast, it was partially abolished in SCCVII cells. These results therefore suggest that the treatment of the cells with MP induced radiosensitization via the production of excess mitochondria-derived ROS in tumor cells.; (主査) 教授 稲波 修, 教授 石塚 真由美, 准教授 佐藤 耕太, 准教授 山盛 徹; 獣医学研究科（獣医学専攻）
• DOI: 10.14943/doctoral.k11283; https://doi.org/10.14943/doctoral.k11283
• 国立国会図書館永続的識別子: info:ndljp/pid/8950082; https://dl.ndl.go.jp/pid/8950082
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 公開開始日（W3CDTF）: 2015-03-03
• 受理日（W3CDTF）: 2015-02-03T05:25:05+09:00
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://dx.doi.org/10.14943/doctoral.k11283; http://hdl.handle.net/2115/56134
• 参照（URI）: http://hdl.handle.net/2115/55307
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• 要約等: Considerable interest has recently been focused on the special characteristics of cancer metabolism, and several drugs designed to modulate cancer met...