がん細胞におけるミトコンドリア由来活性酸素を介した3-Methyl pyruvateによる放射線感受性の増強

• Material Type: 博士論文
• Title: がん細胞におけるミトコンドリア由来活性酸素を介した3-Methyl pyruvateによる放射線感受性の増強
• Author/Editor: 西田, 直哉
• Author Heading: 西田, 直哉
• Publication, Distribution, etc.: Hokkaido University
• Publication Date: 2014-03-25
• Publication Date (W3CDTF): 2014-03-25
• Alternative Title: 3-Methyl pyruvate enhances the radiosensitivity through increase of mitochondria derivedreactive oxygen species in tumor cell lines
• Contributor: 稲波, 修; 石塚, 真由美; 佐藤, 耕太; 山盛, 徹
• Degree grantor/type: 北海道大学
• Date Granted: 2014-03-25
• Date Granted (W3CDTF): 2014-03-25
• Dissertation Number: 甲第11283号
• Degree Type: 博士(獣医学)
• Conferring No. (Dissertation): 甲第11283号
• Text Language Code: jpn
• Alias of Author: Nishida, Naoya
• NDC: 649
• Target Audience: 一般
• Note (General): Considerable interest has recently been focused on the special characteristics of cancer metabolism, and several drugs designed to modulate cancer metabolism have been tested as potential anticancer agents. To date, however, very few studies have been conducted to investigate the combined effects of anticancer drugs and radiotherapy. In this study, to evaluate the role of mitochondria-derived reactive oxygen species (ROS) in the radiation-induced cell death of tumor cells, we have examined the effect of 3-methyl pyruvate (MP). MP is a membrane-permeable pyruvate derivative that is capable of activating mitochondrial energy metabolism, on human lung carcinoma A549 cells and murine squamous carcinoma SCCVII cells. Pretreatment with MP significantly enhanced radiation-induced cell death in both cell lines, and also led to increases in themitochondrial membrane potential, intracellular adenosine triphosphate content, and mitochondria-derived ROS production following the exposure of the cells to X-rays. In A549 cells, MP-induced radiosensitization was completely abolished by vitamin C. In contrast, it was partially abolished in SCCVII cells. These results therefore suggest that the treatment of the cells with MP induced radiosensitization via the production of excess mitochondria-derived ROS in tumor cells.; (主査) 教授 稲波 修, 教授 石塚 真由美, 准教授 佐藤 耕太, 准教授 山盛 徹; 獣医学研究科（獣医学専攻）
• DOI: 10.14943/doctoral.k11283; https://doi.org/10.14943/doctoral.k11283
• Persistent ID (NDL): info:ndljp/pid/8950082; https://dl.ndl.go.jp/pid/8950082
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Available (W3CDTF): 2015-03-03
• Date Accepted (W3CDTF): 2015-02-03T05:25:05+09:00
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.14943/doctoral.k11283; http://hdl.handle.net/2115/56134
• Is Referenced By (URI): http://hdl.handle.net/2115/55307
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Considerable interest has recently been focused on the special characteristics of cancer metabolism, and several drugs designed to modulate cancer met...