Existence of Two Kinds of Sulfur-reducing Systems in Iron-oxidizing Bacterium Thiobacillus ferrooxidans

• Material Type: 記事
• Title: Existence of Two Kinds of Sulfur-reducing Systems in Iron-oxidizing Bacterium Thiobacillus ferrooxidans
• Author/Editor: Kim Yong Ng; Sayuri Inoue; Akira Fujioka 他
• Author Heading: Kim Yong Ng; Sayuri Inoue; Akira Fujioka
• Periodical title: Bioscience, Biotechnology, and Biochemistry
• No. or year of volume/issue: 63(5) (通号 716) 1999.05
• Volume: 63
• Issue: 5
• Sequential issue number: 716
• Pages: 813～819
• Publication date of volume/issue (W3CDTF): 1999-05
• ISSN (Periodical Title): 0916-8451
• ISSN-L (Periodical Title): 0916-8451
• Publication (Periodical Title): Tokyo : Japan Society for Bioscience
• Place of Publication (Country Code): JP
• Text Language Code: eng
• NDLC: ZR7
• Target Audience: 一般
• Holding library: 国立国会図書館
• Call No.: Z53-G223
• Data Provider (Database): 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• Bibliographic ID (NDL): 4743777; http://id.ndl.go.jp/bib/4743777
• Bibliographic Record Category (NDL): 632

• Summary, etc.: Intact cells of <i>Thiobacillus ferrooxidans</i> NASF-1 incubated under anaerobic conditions in a reaction mixture containing 0.5% colloidal sulfur produced hydrogen sulfide (H₂S) extracellularly. The amount of H₂S produced by cells increased corresponding to the cell amounts and colloidal sulfur. Two activity peaks of H₂S production were observed at pH 1.5 and 7.5. We tentatively called the enzyme activities pH 1.5- and pH 7.5-sulfur reducing systems, respectively. Seven strains of <i>T. ferrooxidans</i> tested had both the activities of pH 1.5- and pH 7.5-sulfur reducing systems, but at different levels. <i>T. ferrooxidans</i> NASF-1 showed the highest activity of the pH 1.5-sulfur reducing system and strain 13598 from ATCC showed the highest activity of the pH 7.5-sulfur reducing system. Further characteristics of H₂S production were studied with intact cells of NASF-1. The optimum temperatures for pH 1.5- and pH 7.5-sulfur reducing systems of NASF-1 were 40°C. Hydrogen sulfide production continued for 8 days and total amounts of H₂S produced at pH 7.5 and 1.5 were 832 and 620 nmol/mg protein, respectively. The pH 7.5-sulfur reducing system used only colloidal sulfur as the electron acceptor. However, the pH 1.5-sulfur reducing system used both colloidal sulfur and tetrathionate. Thiosulfate, dithionate, and sulfite could not be used as the electron acceptor for both of the sulfur reducing systems. Potassium cyanide activated by 3- fold the pH 1.5-sulfur reducing system activity at 0.5 m<small>M</small> but did not affect the activity of the pH 7.5-sulfur reducing system. An inhibitor of sulfite reductase, <i>p</i>-chloromercuribenzene sulfonic acid, did not affect either enzyme activity. Sodium molybdate and monoiodoacetic acid strongly inhibited the activity of the pH 1.5-sulfur reducing system at 1.0 m<small>M</small>, but not the activity of pH 7.5-sulfur reducing system.<br>
• DOI: 10.1271/bbb.63.813
• Access Restrictions: インターネット公開
• Data Provider (Database): 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• Summary, etc.: Intact cells of <i>Thiobacillus ferrooxidans</i> NASF-1 incubated under anaerobic conditions in a reaction mixture containing 0.5% colloidal sulfur produced hydrogen sulfide (H₂S) extracellularly. The amount of H₂S produced by cells increased corresponding to the cell amounts and colloidal sulfur. Two activity peaks of H₂S production were observed at pH 1.5 and 7.5. We tentatively called the enzyme activities pH 1.5- and pH 7.5-sulfur reducing systems, respectively. Seven strains of <i>T. ferrooxidans</i> tested had both the activities of pH 1.5- and pH 7.5-sulfur reducing systems, but at different levels. <i>T. ferrooxidans</i> NASF-1 showed the highest activity of the pH 1.5-sulfur reducing system and strain 13598 from ATCC showed the highest activity of the pH 7.5-sulfur reducing system. Further characteristics of H₂S production were studied with intact cells of NASF-1. The optimum temperatures for pH 1.5- and pH 7.5-sulfur reducing systems of NASF-1 were 40°C. Hydrogen sulfide production continued for 8 days and total amounts of H₂S produced at pH 7.5 and 1.5 were 832 and 620 nmol/mg protein, respectively. The pH 7.5-sulfur reducing system used only colloidal sulfur as the electron acceptor. However, the pH 1.5-sulfur reducing system used both colloidal sulfur and tetrathionate. Thiosulfate, dithionate, and sulfite could not be used as the electron acceptor for both of the sulfur reducing systems. Potassium cyanide activated by 3- fold the pH 1.5-sulfur reducing system activity at 0.5 m<small>M</small> but did not affect the activity of the pH 7.5-sulfur reducing system. An inhibitor of sulfite reductase, <i>p</i>-chloromercuribenzene sulfonic acid, did not affect either enzyme activity. Sodium molybdate and monoiodoacetic acid strongly inhibited the activity of the pH 1.5-sulfur reducing system at 1.0 m<small>M</small>, but not the activity of pH 7.5-sulfur reducing system.<br>
• DOI: 10.1271/bbb.63.813; https://doi.org/10.1271/bbb.63.813
• Related Material (URI): http://id.ndl.go.jp/bib/4743777; https://ndlsearch.ndl.go.jp/books/R000000004-I4743777; http://www.tandfonline.com/doi/pdf/10.1271/bbb.63.813
• Is Referenced By: Purification and Some Properties of Sulfur Reductase from the Iron-Oxidizing Bacterium Thiobacillus ferrooxidans NASF-1.; https://cir.nii.ac.jp/crid/1360283694137054080; Production of hydrogen sulfide from tetrathionate by the iron-oxidizing bacterium Thiobacillus ferrooxidans NASF-1; https://cir.nii.ac.jp/crid/1360284919608015872
• Data Provider (Database): 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• Original Data Provider (Database): Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• Bibliographic ID (NDL): 4743777; http://id.ndl.go.jp/bib/4743777
• NAID: 110002679569