Existence of Two Kinds of Sulfur-reducing Systems in Iron-oxidizing Bacterium Thiobacillus ferrooxidans

• 資料種別: 記事
• タイトル: Existence of Two Kinds of Sulfur-reducing Systems in Iron-oxidizing Bacterium Thiobacillus ferrooxidans
• 著者・編者: Kim Yong Ng; Sayuri Inoue; Akira Fujioka 他
• 著者標目: Kim Yong Ng; Sayuri Inoue; Akira Fujioka
• タイトル（掲載誌）: Bioscience, Biotechnology, and Biochemistry
• 巻号年月日等（掲載誌）: 63(5) (通号 716) 1999.05
• 掲載巻: 63
• 掲載号: 5
• 掲載通号: 716
• 掲載ページ: 813～819
• 掲載年月日（W3CDTF）: 1999-05
• ISSN（掲載誌）: 0916-8451
• ISSN-L（掲載誌）: 0916-8451
• 出版事項（掲載誌）: Tokyo : Japan Society for Bioscience
• 出版地（国名コード）: JP
• 本文の言語コード: eng
• NDLC: ZR7
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z53-G223
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 4743777; http://id.ndl.go.jp/bib/4743777
• 整理区分コード: 632

• 要約等: Intact cells of <i>Thiobacillus ferrooxidans</i> NASF-1 incubated under anaerobic conditions in a reaction mixture containing 0.5% colloidal sulfur produced hydrogen sulfide (H₂S) extracellularly. The amount of H₂S produced by cells increased corresponding to the cell amounts and colloidal sulfur. Two activity peaks of H₂S production were observed at pH 1.5 and 7.5. We tentatively called the enzyme activities pH 1.5- and pH 7.5-sulfur reducing systems, respectively. Seven strains of <i>T. ferrooxidans</i> tested had both the activities of pH 1.5- and pH 7.5-sulfur reducing systems, but at different levels. <i>T. ferrooxidans</i> NASF-1 showed the highest activity of the pH 1.5-sulfur reducing system and strain 13598 from ATCC showed the highest activity of the pH 7.5-sulfur reducing system. Further characteristics of H₂S production were studied with intact cells of NASF-1. The optimum temperatures for pH 1.5- and pH 7.5-sulfur reducing systems of NASF-1 were 40°C. Hydrogen sulfide production continued for 8 days and total amounts of H₂S produced at pH 7.5 and 1.5 were 832 and 620 nmol/mg protein, respectively. The pH 7.5-sulfur reducing system used only colloidal sulfur as the electron acceptor. However, the pH 1.5-sulfur reducing system used both colloidal sulfur and tetrathionate. Thiosulfate, dithionate, and sulfite could not be used as the electron acceptor for both of the sulfur reducing systems. Potassium cyanide activated by 3- fold the pH 1.5-sulfur reducing system activity at 0.5 m<small>M</small> but did not affect the activity of the pH 7.5-sulfur reducing system. An inhibitor of sulfite reductase, <i>p</i>-chloromercuribenzene sulfonic acid, did not affect either enzyme activity. Sodium molybdate and monoiodoacetic acid strongly inhibited the activity of the pH 1.5-sulfur reducing system at 1.0 m<small>M</small>, but not the activity of pH 7.5-sulfur reducing system.<br>
• DOI: 10.1271/bbb.63.813
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: Intact cells of <i>Thiobacillus ferrooxidans</i> NASF-1 incubated under anaerobic conditions in a reaction mixture containing 0.5% colloidal sulfur produced hydrogen sulfide (H₂S) extracellularly. The amount of H₂S produced by cells increased corresponding to the cell amounts and colloidal sulfur. Two activity peaks of H₂S production were observed at pH 1.5 and 7.5. We tentatively called the enzyme activities pH 1.5- and pH 7.5-sulfur reducing systems, respectively. Seven strains of <i>T. ferrooxidans</i> tested had both the activities of pH 1.5- and pH 7.5-sulfur reducing systems, but at different levels. <i>T. ferrooxidans</i> NASF-1 showed the highest activity of the pH 1.5-sulfur reducing system and strain 13598 from ATCC showed the highest activity of the pH 7.5-sulfur reducing system. Further characteristics of H₂S production were studied with intact cells of NASF-1. The optimum temperatures for pH 1.5- and pH 7.5-sulfur reducing systems of NASF-1 were 40°C. Hydrogen sulfide production continued for 8 days and total amounts of H₂S produced at pH 7.5 and 1.5 were 832 and 620 nmol/mg protein, respectively. The pH 7.5-sulfur reducing system used only colloidal sulfur as the electron acceptor. However, the pH 1.5-sulfur reducing system used both colloidal sulfur and tetrathionate. Thiosulfate, dithionate, and sulfite could not be used as the electron acceptor for both of the sulfur reducing systems. Potassium cyanide activated by 3- fold the pH 1.5-sulfur reducing system activity at 0.5 m<small>M</small> but did not affect the activity of the pH 7.5-sulfur reducing system. An inhibitor of sulfite reductase, <i>p</i>-chloromercuribenzene sulfonic acid, did not affect either enzyme activity. Sodium molybdate and monoiodoacetic acid strongly inhibited the activity of the pH 1.5-sulfur reducing system at 1.0 m<small>M</small>, but not the activity of pH 7.5-sulfur reducing system.<br>
• DOI: 10.1271/bbb.63.813; https://doi.org/10.1271/bbb.63.813
• 関連情報（URI）: http://id.ndl.go.jp/bib/4743777; https://ndlsearch.ndl.go.jp/books/R000000004-I4743777; http://www.tandfonline.com/doi/pdf/10.1271/bbb.63.813
• 参照: Purification and Some Properties of Sulfur Reductase from the Iron-Oxidizing Bacterium Thiobacillus ferrooxidans NASF-1.; https://cir.nii.ac.jp/crid/1360283694137054080; Production of hydrogen sulfide from tetrathionate by the iron-oxidizing bacterium Thiobacillus ferrooxidans NASF-1; https://cir.nii.ac.jp/crid/1360284919608015872
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• 書誌ID（NDLBibID）: 4743777; http://id.ndl.go.jp/bib/4743777
• NII論文ID: 110002679569