コヒーレントＸ線回折による酵母核内の核酸分布イメージング

Material type: 文書・図像類

Author: 中迫, 雅由

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Publication date: 2018

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Note (General)：: type:textシンクロトロン放射光を用いた低温Ｘ線回折イメージング・トモグラフィーと、Ｘ線自由電子レーザーを用いた低温Ｘ線回折イメージングにおける試料作製および実験装置の高度化をおこなうとともに、新たな構造解析法を考案した。それらをもとに、バクテリア細胞や酵母細胞核の普遍的な内部構造などを可視化...

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科研費研究者番号 : 30227764

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Material Type: 文書・図像類
Title: コヒーレントＸ線回折による酵母核内の核酸分布イメージング
Author/Editor: 中迫, 雅由
Author Heading: 中迫, 雅由
Publication Date: 2018
Publication Date (W3CDTF): 2018
Alternative Title: コヒーレント Xセンカイセツニヨルコウボカクナイノカクサンブンプイメージング
Kohīrento Xsen kaisetsu ni yoru kōbo kakunai no kakusan bunpu imējingu
Visualization on the distribution of nucreic acids in yeast nucleus by using coherent X-ray diffraction
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: Ｘ線回折イメージング,放射光,Ｘ線自由電子レーザー,低温Ｘ線回折実験,位相回復,三次元再構成,細胞核,細胞非侵襲イメージング
Target Audience: 一般
Note (General): type:text
シンクロトロン放射光を用いた低温Ｘ線回折イメージング・トモグラフィーと、Ｘ線自由電子レーザーを用いた低温Ｘ線回折イメージングにおける試料作製および実験装置の高度化をおこなうとともに、新たな構造解析法を考案した。それらをもとに、バクテリア細胞や酵母細胞核の普遍的な内部構造などを可視化するとともに、バクテリア細胞や酵母細胞の三次元構造解析を行い、生体非結晶粒子の非侵襲イメージングを確立した。 In this project, we improved fundamental techniques including automatic control procedures of diffraction apparatuses, preparation of frozen-hydrated biological specimens, and measurement of spatial coherence of X-ray beam, in cryogenic X-ray diffraction imaging tomography using synchrotron X-rays and cryogenic X-ray diffraction using X-ray free electron laser pulses. In addition, we developed methods to obtain reliable electron density maps in phase retrieval calculation in structure analyses including the utilization of manifold learning. Based on these developments, we successfully established X-ray diffraction imaging technique for non-crystalline biological specimens, such as cells and organelles, through the visualization of common structures in yeast nucleus and cyanobacterium cell as well as the three-dimensional structures of yeast and bacteria cells without sectioning and chemical labeling necessary in other imaging techniques.
研究種目 : 基盤研究 (A) (一般) 研究期間 : 2016～2018 課題番号 : 16H02218 研究分野 : 生物物理
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 30227764
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ