概日リズムをモデル系に新規ポリA鎖長決定法を用いた翻訳制御プラットフォームの構築

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Author: 程, 肇ほか

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Publication date: 2015-06-15

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Note (General)：: 金沢大学理工研究域生命理工学系一般にmRNA のポリA 鎖長は不均一な分布をもち、その長さを簡便にかつ厳密に決定できる方法は現在ない。そこで従来のAnchored RT-PCR 法を改良して、PACHINCO (Poly(A) Capture by Hairpin Chimeric Oligonuc...

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https://kaken.nii.ac.jp/search/?qm=00242115

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25640100/

https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25640100/25640100seika/

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Material Type: 文書・図像類
Title: 概日リズムをモデル系に新規ポリA鎖長決定法を用いた翻訳制御プラットフォームの構築
Author/Editor: 程, 肇
Tei, Hajime
Author Heading: 程, 肇
Tei, Hajime
Publication Date: 2015-06-15
Publication Date (W3CDTF): 2015-06-15
Alternative Title: Development of translational regulation platform of circadian rhythms using novel poly(A) determination method
Periodical title: 平成26(2014)年度科学研究費補助金挑戦的萌芽研究研究成果報告書 = 2014 Fiscal Year Final Research Report
No. or year of volume/issue: 2013-04-01 - 2015-03-31
Volume: 2013-04-01 - 2015-03-31
Pages: 4p.-
Text Language Code: jpn
Subject Heading: 概日リズム
時計遺伝子
視交叉上核
翻訳制御
Period1
poly(A)
Target Audience: 一般
Note (General): 金沢大学理工研究域生命理工学系
一般にmRNA のポリA 鎖長は不均一な分布をもち、その長さを簡便にかつ厳密に決定できる方法は現在ない。そこで従来のAnchored RT-PCR 法を改良して、PACHINCO (Poly(A) Capture by Hairpin Chimeric Oligonucleotide)-RT-PCR 法を構築した。この方法は遺伝子特異的5’PCR プライマーをゲノム全遺伝子のmRNA 3’UTR に対応させるだけで、全mRNA ポリA 鎖長をハイスループットに決定できる。実際、全自動型DNA 分析用マイクロチップ電気泳動装置を用いて、Per1 mRNA のポリA 鎖伸長を確認することができた。
In general, the length of poly (A) in eukaryotic mRNA shows a wide distribution, and at present, there is no precise and simple method to determine it. A novel method for its determination was developed with the improvement of conventional anchored RT-PCR, and designated as PACHINCO (Poly (A) Capture by Hairpin Chimeric Oligonucleotide)-RT-PCR. The new method can be applied for the comprehensive determination of poly (A) in whole cellular mRNAs with simply replacing 5' primers used for PACHINCO-RT-PR with sequences involved in the corresponding 3'UTRs of mRNA. Indeed, the method was optimized for an automatic microchip DNA electrophoresis apparatus, and the elongation of poly (A) of Per1 mRNA was identified using this system.
研究課題/領域番号:25640100, 研究期間(年度):2013-04-01 - 2015-03-31
DOI: 10.24517/00051934
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=00242115
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25640100/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25640100/25640100seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA