新規ポリA鎖長決定法を用いた概日リズムが見られるmRNAのゲノムワイド検索

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Author: 程, 肇ほか

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Publication date: 2012-04-01

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Note (General)：: 金沢大学理工研究域生命理工学系mRNAのポリA鎖長を決定する、PACHINCO(Poly(A) Capture by Hairpin ChimericOligonucleotide)-RT-PCR法を構築した。本年度は本方法を全自動型DNA分析用マイクロチップ電気泳動装置(Shimadzu社)に適用...

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https://kaken.nii.ac.jp/search/?qm=00242115

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651184/

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Material Type: 文書・図像類
Title: 新規ポリA鎖長決定法を用いた概日リズムが見られるmRNAのゲノムワイド検索
Author/Editor: 程, 肇
Tei, Hajime
Author Heading: 程, 肇
Tei, Hajime
Publication Date: 2012-04-01
Publication Date (W3CDTF): 2012-04-01
Alternative Title: A Genome-wide screening of circadian transcripts using a novel poly(A) determination method
Periodical title: 平成23(2011)年度科学研究費補助金挑戦的萌芽研究研究成果報告書 = 2011 Fiscal Year Final Research Report
No. or year of volume/issue: 2011
Volume: 2011
Pages: 4p.-
Text Language Code: jpn
Subject Heading: 概日リズム
時計遺伝子
視交叉上核
Periodl
poly(A)
翻訳制御
Target Audience: 一般
Note (General): 金沢大学理工研究域生命理工学系
mRNAのポリA鎖長を決定する、PACHINCO(Poly(A) Capture by Hairpin ChimericOligonucleotide)-RT-PCR法を構築した。本年度は本方法を全自動型DNA分析用マイクロチップ電気泳動装置(Shimadzu社)に適用するための条件の最適化を実施した。その結果、poly(A-T)鎖を効率的に検出する泳動条件を決定し、さらに、自動電気泳動装置からの出力データを用いたpoly(A)鎖長分布解析プログラムを開発した。
We have developed a novel method to determine the length of poly(A) in eukaryotic mRNA, and designated as PACHINCO(Poly(A) Capture by Hairpin Chimeric Oligonucleotide)-RT-PCR. In this year, we optimized the application of the method to an automated microchip electrophoresis system(MultiNA, SHIMADZU), including the development of the distribution analysis algorithm of a variety of poly(A) length.
研究課題/領域番号:23651184, 研究期間(年度):2011
出典：研究課題「新規ポリA鎖長決定法を用いた概日リズムが見られるmRNAのゲノムワイド検索」課題番号23651184（KAKEN：科学研究費助成事業データベース（国立情報学研究所））（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23651184/23651184seika/)を加工して作成
DOI: 10.24517/00051936
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=00242115
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651184/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23651184/23651184seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA