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- Material Type
- 文書・図像類
- Title
- Author/Editor
- 吉田, 光敏
- Author Heading
- 吉田, 光敏 ヨシダ, ミツトシ
- Publication, Distribution, etc.
- Alternative Title
- Improvement of the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells using a histone deacetylase inhibitor and latrunculin A
- Text Language Code
- jpn
- Target Audience
- 一般
- Note (General)
- 2008-2010年度科学研究費補助金(基盤研究(C))研究成果報告書 課題番号：20580311 研究代表者：吉田光敏 (鹿児島大学農学部教授)遺伝子導入クラウン系ミニブタ体細胞クローン胚の体外発生・体内発生に及ぼす脱アセチル化酵素阻害剤処理(HADCi)および細胞骨格阻害剤処理の影響について検討した。その結果、体細胞クローン胚の胚盤胞への体外発生能の超高度化にはHDACi処理(トリコスタチンA、スクリパタイドまたはスベロイルビスヒドロキサム酸)および細胞骨格阻害剤(ラトランキュリンA)処理が有効であり、得られた体細胞クローン胚の胎仔および産仔への体内発生能が示された。The objective of this study was to examine the effects of postactivation treatment of histone deacetylase inhibitors (HADCi) and an actin polymerisation inhibitor,latrunculin A (LatA) on in vitro and in vivo development of somatic cell nuclear transfer (SCNT) embryos derived from gene^modified Clawn miniature pig cells. After electric activation, SCNT embryos were treated with or without LatA, and cultured in vitro. The rate of blastocyst formation was significantly higher (p<0.05) in SCNT embryos treated with HADCi and 0.5μM LatA than that in control. In addition, fetuses and piglets were obtained from recipient uteri after transfer of embryos treated with HDACi and LatA. The results of this study showed for the first time that postactivation treatment with HDACi and LatA was effective to improve in vitro and in vivo developmental capacity of gene-modified cloned miniature pig embryos.