硬骨魚類由来カルシウム代謝調節ホルモン・スタニオカルシンの骨代謝調節作用

• Material Type: 博士論文
• Title: 硬骨魚類由来カルシウム代謝調節ホルモン・スタニオカルシンの骨代謝調節作用
• Author/Editor: 吉子, 裕二
• Author Heading: 吉子, 裕二
• Alternative Title: Effects of chum salmon stanniocalcin,a calcium regulatiug hormone in teleosts and its synthetic N-terminal peptide fragment on bone metabolism
• Degree grantor/type: 広島大学
• Date Granted: 1996-03-06
• Dissertation Number: 乙第2836号
• Degree Type: 博士(歯学); Dentistry
• Text Language Code: eng
• Target Audience: 一般
• DOI: 10.11501/3120004
• Format (IMT): application/pdf
• Source: http://ir.lib.hiroshima-u.ac.jp/31885/files/28517 （fulltext）; http://ir.lib.hiroshima-u.ac.jp/31885/files/28517
• Access Restrictions: インターネット公開
• Rights (production): Copyright(c) by Author
• References: Yoshiko, Y., Kosugi, T. and Koide, Y. Effects of a synthetic N-terminal fragment of stanniocalcin on the metabolism of mammalian bone in vitro. Biochimica et Biophysica Acta, in press.
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 広島大学 : 広島大学学術情報リポジトリ

Table of Contents

CONTENTS
1. INTRODUCTION / p1
2. MATERIALS AND METHODS / p4
　2.1. Preparation of chum salmon stanniocalcin (STC) / p4
　2.2. Intestinal Ca²⁺influx of Atlantic cod / p4
　2.3. Preparation of peptide fragments of STC / p5
　2.4. Amino acid sequence analysis / p5
　2.5. Cloning of cDNA encoding STC / p6
　2.6. Synthesis of N-terminal peptide fragment of STC (peptide N) / p7
　2.7. Calvarial bone resorption assay / p7
　2.8. Generation of osteoclast-like cells / p8
　2.9. Production of cyclic AMP in osteoblast-like cells / p8
　2.10. Synthesis of collagen in calvarial bone / p9
　2.11. Statistical analysis / p9
3. RESULTS / p10
　3.1. Isolation and characterization of STC / p10
　3.2. Amino acid and cDNA sequence analysis of STC / p11
　3.3. Synthesis of peptide N / p12
　3.4. Effects of STC and peptide N on the calvarial bone resorption / p12
　3.5. Effects of STC and peptide N on generation of osteoclast-like cells / p13
　3.6. Effects of peptide N on the production of cyclic AMP in osteoblast-like cells / p14
　3.7. Effects of peptide N on the synthesis of calvarial bone collagen / p14
4. DISCUSSION / p16
　4.1. Chemical properties of STC / p16
　4.2. Effects of STC and peptide N on the metabolism of mammalian bone / p18
5. SUMMARY / p23
6. REFERENCES / p25