Construction of hybrid molecule libraries based on thioetherification of T7 phage-displayed peptides

Persistent ID (NDL): info:ndljp/pid/10120619

Material type: 博士論文

Author: 福永, 圭佑ほか

Publisher: The University of Electro-Communications

Publication date: 2014-03-24

Material Format: Digital

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Name of awarding university/degree: 電気通信大学,博士（工学）

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Note (General)：: Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide. Commercially available pre-made phage libra...

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Material Type: 博士論文
Title: Construction of hybrid molecule libraries based on thioetherification of T7 phage-displayed peptides
Author/Editor: 福永, 圭佑
Keisuke, Fukunaga
Author Heading: 福永, 圭佑
Keisuke, Fukunaga
Publication, Distribution, etc.: The University of Electro-Communications
Publication Date: 2014-03-24
Publication Date (W3CDTF): 2014-03-24
Alternative Title: T7ファージ提示ペプチドのチオエーテル化を基盤としたハイブリッド分子ライブラリーの作製
Periodical title: 学位論文
Degree grantor/type: 電気通信大学
Date Granted: 2014-03-24
Date Granted (W3CDTF): 2014-03-24
Dissertation Number: 甲第767号
Degree Type: 博士（工学）
Conferring No. (Dissertation): 12612甲第767号
Text Language Code: eng
Target Audience: 一般
Note (General): Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide. Commercially available pre-made phage libraries allow us to take screening in the easiest way. On the other hand, construction of a custom phage library seems to be inaccessible, because several practical tips are absent in instructions. This chapter focuses on what should be born in mind for beginners using commercially available cloning kits (i.e., Ph.D. with type 3 vector and T7Select systems for M13 and T7 phage, respectively). In the M13 system, proline or basic amino acids (especially, Arg) should be avoided at the N-terminus of peptide fused to gp3. In both systems, peptides containing odd number(s) of cysteine(s) should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.
2013
Persistent ID (NDL): info:ndljp/pid/10120619
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Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2016-07-07T04:28:02+09:00
Format (IMT): application/pdf
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Periodical Title (URI): http://id.nii.ac.jp/1438/00000936/
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