Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice

• Material Type: 博士論文
• ISSN: 0142-9612
• Title: Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice
• Volume: 65
• Author/Editor: 堺, 裕輔
• Author Heading: 堺, 裕輔
• Publication, Distribution, etc.: Elsevier Ltd.
• Publication Date: 2016-08-03
• Publication Date (W3CDTF): 2016-08-03
• Alternative Title: 肝細胞／線維芽細胞複合シートによる血管誘導皮下性ヒト肝組織の構築
• Periodical title: Biomaterials
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2016-08-03
• Date Granted (W3CDTF): 2016-08-03
• Dissertation Number: 乙医歯薬第45号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 乙医歯薬第45号
• Text Language Code: eng
• Subject Heading: Cell sheet; Fibroblast; Hepatocyte transplantation; Human primary hepatocyte; Tissue engineering; Vascularization
• Target Audience: 一般
• Note (General): Subcutaneous liver tissue engineering is an attractive and minimally invasive approach used to curative treat hepatic failure and inherited liver diseases. However, graft failure occurs frequently due to insufficient infiltration of blood vessels (neoangiogenesis), while the maintenance of hepatocyte phenotype and function requires in vivo development of the complex cellular organization of the hepatic lobule. Here we describe a subcutaneous human liver construction allowing for rapidly vascularized grafts by transplanting engineered cellular sheets consisting of human primary hepatocytes adhered onto a fibroblast layer. The engineered hepatocyte/fibroblast sheets (EHFSs) showed superior expression levels of vascularization-associated growth factors (vascular endothelial growth factor, transforming growth factor beta 1, and hepatocyte growth factor) in vitro. EHFSs developed into vascularized subcutaneous human liver tissues contained glycogen stores, synthesized coagulation factor IX, and showed significantly higher synthesis rates of liver-specific proteins (albumin and alpha 1 anti-trypsin) in vivo than tissues from hepatocyte-only sheets. The present study describes a new approach for vascularized human liver organogenesis under mouse skin. This approach could prove valuable for establishing novel cell therapies for liver diseases.; 長崎大学学位論文 学位記番号:博(医歯薬)乙第45号 学位授与年月日:平成28年8月3日; Author: Yusuke Sakai, Kosho Yamanouchi, Kazuo Ohashi, Makiko Koike, Rie Utoh, Hideko Hasegawa, Izumi Muraoka, Takashi Suematsu, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi; Citation: Biomaterials, 65, pp.66-75; 2015; Nagasaki University (長崎大学), 博士(医学) (2016-08-03)
• DOI: info:doi/10.1016/j.biomaterials.2015.06.046; https://doi.org/info:doi/10.1016/j.biomaterials.2015.06.046
• Persistent ID (NDL): info:ndljp/pid/10192297; https://dl.ndl.go.jp/pid/10192297
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-09-02T08:39:13+09:00
• Date Created (W3CDTF): 2020-12-21
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10069/36670; https://nagasaki-u.repo.nii.ac.jp/records/3505
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2021-03-07 再収集