網膜虚血再灌流障害における分散ヘスペレチンの神経保護効果 60 1

The cover of this title could differ from library to library.

Available in National Diet Library

Find on the publisher's website

国立国会図書館デジタルコレクション

Digital data available

Check on the publisher's website

DOI[info:doi/10.1007/s10384-015-0415-z]to the data of the same series

網膜虚血再灌流障害における分散ヘスペレチンの神経保護効果 1

Persistent ID (NDL): info:ndljp/pid/10211583

Material type: 博士論文

Author: 下内, 昭人

Publisher: -

Publication date: 2016-01

Material Format: Digital

Capacity, size, etc.: -

Name of awarding university/degree: 旭川医科大学,博士（医学）

View All

Notes on use at the National Diet Library

本資料は、掲載誌(URI)等のリンク先にある学位授与機関のWebサイトやCiNii Dissertationsから、本文を自由に閲覧できる場合があります。

Notes on use

Note (General)：: First online: 25 September 2015 The final publication is available at Springer via http://dx.doi.org/10.1007/s10384-015-0415-zPurpose To determine w...

Search by Bookstore

Read this material in an accessible format.

View materials in accessible formats for people with print disabilities (1Type)

Search by Bookstore

Read in Disability Resources

Other Services
- テキストデータ check it on 国立国会図書館デジタルコレクション

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 博士論文
ISSN: 0021-5155
Title: 網膜虚血再灌流障害における分散ヘスペレチンの神経保護効果
Volume: 60 1
Author/Editor: 下内, 昭人
Publication Date: 2016-01
Publication Date (W3CDTF): 2016-01
Alternative Title: Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury
Periodical title: Japanese Journal of Ophthalmology
Degree grantor/type: 旭川医科大学
Date Granted: 2016-03-25
Date Granted (W3CDTF): 2016-03-25
Dissertation Number: 甲第492号
Degree Type: 博士（医学）
Conferring No. (Dissertation): 甲第492号
Text Language Code: eng
Subject Heading: Hesperetin
Ischemia–reperfusion
Neuroprotection
Retina
Inflammation
Note (General): First online: 25 September 2015 The final publication is available at Springer via http://dx.doi.org/10.1007/s10384-015-0415-z
Purpose To determine whether water-dispersible hesperetin (WD-Hpt) can prevent degeneration of ganglion cell neurons in the ischemic retina. Methods Ischemia reperfusion (I/R) injury was induced by increasing the intraocular pressure of mice to 110 mmHg for 40 min. Mice received daily intraperitoneal injections with either normal saline (NS, 0.3 ml/day) or WD-Hpt (0.3 ml, 200 mg/kg/day). Reactive oxygen species (ROS) was assessed by dihydroethidium and nitrotyrosine formation. Inflammation was estimated by microglial morphology in the retina. Lipopolysaccharide (LPS)-stimulated BV-2 cells were used to explore the anti-inflammatory effect of WD-Hpt on activated microglia by quantifying the expression of IL-1β using real-time quantitative reverse transcription-polymerase chain reaction. Ganglion cell loss was assessed by immunohistochemistry of NeuN. Glial activation was quantified with glial fibrillary acidic protein (GFAP) immunoreactivity. Apoptosis was evaluated with a terminal deoxynucleotidyl transferase (TUNEL) assay and immunohistochemistry of cleaved caspase-3. Phosphorylation of extracellular signal-regulated kinase (p-ERK) was surveyed by western blotting. Results WD-Hpt decreased I/R-induced ROS formation. WD-Hpt alleviated microglial activation induced by I/R and reduced mRNA levels of IL-1β in LPS-stimulated BV-2. I/R resulted in a 37 % reduction in the number of ganglion cells in the NS-treated mice, whereas the reduction was only 5 % in the WD-Hpt-treated mice. In addition, WD-Hpt mitigated the immunoreactivity of GFAP, increased expression of cleaved caspase-3, increased number of TUNEL positive cells and p-ERK after I/R. Conclusions WD-Hpt protected ganglion cells from I/R injury by inhibiting oxidative stress and modulating cell death signaling. Moreover, WD-Hpt had an anti-inflammatory effect through the suppression of activated microglia.
identifier:PMID:26407617
開始ページ : 51
終了ページ : 61
DOI: info:doi/10.1007/s10384-015-0415-z
https://doi.org/info:doi/10.1007/s10384-015-0415-z
Persistent ID (NDL): info:ndljp/pid/10211583
https://dl.ndl.go.jp/pid/10211583
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2016-11-02T12:02:18+09:00
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): http://amcor.asahikawa-med.ac.jp/modules/xoonips/detail.php?id=20160325_K492
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
https://dl.ndl.go.jp