Flow Cytometric Evaluation of Surface CD56 Expression on Activated Natural Killer Cells as Functional Marker

• Material Type: 博士論文
• ISSN: 1349-6867
• Title: Flow Cytometric Evaluation of Surface CD56 Expression on Activated Natural Killer Cells as Functional Marker
• Volume: 63 3-4
• Author/Editor: Oboshi, Wataru; Aki, Kensaku; Tada, Tomoki; Watanabe, Toru; Yukimasa, Nobuyasu; Ueno, Ichiro; Saito, Ken; Hosoi, Eiji
• Author Heading: Oboshi, Wataru; Aki, Kensaku; Tada, Tomoki; Watanabe, Toru; Yukimasa, Nobuyasu; Ueno, Ichiro; Saito, Ken; Hosoi, Eiji
• Publication, Distribution, etc.: Faculty of Medicine Tokushima University
• Publication Date: 2016-08
• Publication Date (W3CDTF): 2016-08
• Alternative Title: 機能的マーカーとしての活性化NK細胞表面CD56発現のフローサイトメトリー評価; Evaluation of Surface CD56 Expression
• Periodical title: The Journal of Medical Investigation
• Degree grantor/type: 徳島大学
• Date Granted: 2016-09-15
• Date Granted (W3CDTF): 2016-09-15
• Dissertation Number: 甲第2975号
• Degree Type: 博士（保健学）
• Conferring No. (Dissertation): 甲第2975号
• Text Language Code: eng
• Subject Heading: natural killer (NK) cells; CD56; cytotoxicity; IFN-γmRNA; flow cytometry
• Target Audience: 一般
• Note (General): Surface CD56 is the most important cell marker for defining NK cells. However, the relationship between the expression of surface CD56 and NK cell activity has not yet been elucidated in detail. Thirteen healthy volunteers were enrolled in the present study. Peripheral blood mononuclear cells (PBMCs) were stimulated with rIL-2 or rIL-12 (1, 10, 100 U/mL) for 18 h at 37 ℃. After incubation, surface CD56 expression on NK cells was evaluated using a flow cytometric analysis. A colorimetric-based lactate dehydrogenase (LDH) assay was used for experiments on cytotoxicity. IFN-γmRNA gene expression was quantified by real-time PCR. The expression level of surface CD56 on NK cells, cytotoxicity, and IFN-γmRNA gene expression were significantly increased by the rIL-2 and rIL-12 stimulations. In addition, a positive correlation was found between surface CD56 expression and cytotoxic activity or IFN-γmRNA gene expression. We revealed that the quantification of surface CD56 expression was applicable to the evaluation of cytotoxicity and IFN-γproduction in activated NK cells. These results suggest that the measurement of surface CD56 expression represent an easy and rapidly reproducible technique to evaluate the activated state of NK cells and monitor NK cell activity in immunotherapy.
• DOI: info:doi/10.2152/jmi.63.199; https://doi.org/info:doi/10.2152/jmi.63.199
• Persistent ID (NDL): info:ndljp/pid/10224785; https://dl.ndl.go.jp/pid/10224785
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-12-01T22:39:34+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Availability of remote photoduplication service: 可
• Periodical Title (URI): https://repo.lib.tokushima-u.ac.jp/111181
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp