Development of a simple and specific direct competitive ELISA for the determination of artesunate using an anti-artesunate polyclonal antiserum

• Material Type: 記事
• Title: Development of a simple and specific direct competitive ELISA for the determination of artesunate using an anti-artesunate polyclonal antiserum
• Author/Editor: Yoshinori Mitsui
• Publication, Distribution, etc.: 日本 : Springer Nature
• Publication Date: 2016-11-21
• Publication Date (W3CDTF): 2016-11-21
• Periodical title: Tropical medicine and health
• No. or year of volume/issue: 44(37)
• Volume: 44(37)
• ISSN (Periodical Title): 1349-4147
• ISSN-L (Periodical Title): 1348-8945
• Text Language Code: eng
• DOI: 10.1186/s41182-016-0037-2; https://doi.org/10.1186/s41182-016-0037-2
• Persistent ID (NDL): info:ndljp/pid/10233122; https://dl.ndl.go.jp/pid/10233122
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• Acquisition Basis: オンライン資料収集制度
• Date Accepted (W3CDTF): 2016-12-28T13:40:57+09:00
• Date Captured (W3CDTF): 2016-12-26
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• Summary, etc.: Background: Since artesunate (ART) became a vital component of artemisinin (ARM)-based combination therapies for the treatment for malaria,counterfeit ART drugs have spread in regions of Southeast Asia and Africa. The consumption of counterfeit ART drugs has resulted in the death of many patients. Thus,evaluating the quality of ART drugs is needed. There are several methods for quantitating the ART content in tablets,the most common being a high-performance liquid chromatography. However,that method is hampered by the need for expensive equipment and a rather time-consuming process of extraction. By contrast,enzyme-linked immunosorbent assays (ELISAs) are faster and much less expensive,and they require less sample preparation than the above method. The objective of the present study was to establish a simple and specific direct competitive ELISA for the determination of ART concentrations using an anti-ART polyclonal antibody (pAb). Results: Anti-ART pAb was raised in mice,and ART-horseradish peroxidase (HRP) conjugate was produced. A direct competitive ELISA was performed by simultaneously incubating ART and the ART-HRP conjugate with the anti-ART pAb over a second antibody. Subsequently,the enzyme activity of the remaining ART-HRP conjugate was measured. The intra-and inter-assay coefficients of variation of the ELISA were less than 10 % in the range of 0.3 to 30 ng/ml with a detection limit of 0.1 ng/ml. The cross-reactivities of the anti-ART pAb with ARM and dihydroartemisinin were 0.12 and 0.04 %,respectively,and those with other antimalarial drugs were negligible. Furthermore,the recovery of 10 or 50 ng/ml ART added to the drug tablet solutions containing an expected amount of 10 ng/ml was estimated by the ELISA. The recovery of the ART amount ranged between 98 and 106 %,with coefficient variations of less than 7.0 %. Conclusions: The present ELISA is a simple and specific method for the determination of ART concentrations. Thus,this ELISA can be used to identify ART counterfeits and substandard drugs and to quantify the ART drugs.; Tropical Medicine and Health, 44, 37; 2016
• DOI: 10.1186/s41182-016-0037-2; https://doi.org/10.1186/s41182-016-0037-2
• Access Restrictions: インターネット公開
• Rights (production): c The Author(s) 2016. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
• Related Material (URI): http://hdl.handle.net/10069/37352; https://nagasaki-u.repo.nii.ac.jp/records/2154; http://id.ndl.go.jp/digimeta/10233122; https://dl.ndl.go.jp/pid/10233122; http://link.springer.com/content/pdf/10.1186/s41182-016-0037-2.pdf; https://search.jamas.or.jp/link/ui/2018007049
• Is Referenced By: Preparation of Luciferase-fused Peptides for Immunoassay of Amyloid Beta; https://cir.nii.ac.jp/crid/1390287982964190080
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• Bibliographic ID (NDL): 10233122; http://id.ndl.go.jp/bib/10233122
• NAID: 120006987607