Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells.

• Material Type: 博士論文
• Title: Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells.
• Author/Editor: Eino, Atsushi
• Publication, Distribution, etc.: 新潟大学
• Publication Date: 2016-03-23
• Publication Date (W3CDTF): 2016-03-23
• Alternative Title: Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells.; Sqstm1-GFP ノックインマウスが明らかにした、オートファジーとストレス条件でのSqstm1 の動態
• Degree grantor/type: 新潟大学
• Date Granted: 2016-03-23
• Date Granted (W3CDTF): 2016-03-23
• Dissertation Number: 甲第4127号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第4127号
• Text Language Code: eng
• Subject Heading: Sqstm1; Autophagy; Selective autophagy; Nrf2; Knock-in mice
• Note (General): Sqstm1 serves as a signaling hub and receptor for selective autophagy. Consequently, dysregulation of Sqstm1 causes imbalances in signaling pathways and disrupts proteostasis, thereby contributing to the development of human diseases. Environmental stresses influence the level of Sqstm1 by altering its expression and/or autophagic degradation, and also changes the localization of Sqstm1, making it difficult to elucidate the actions and roles of this protein. In this study, we developed knock-in mice expressing Sqstm1 fused to GFP (Sqstm1-GFP^<KI/+>). Using these Sqstm1-GFP^<KI/+> mice, we revealed for the first time the dynamics of endogenous Sqstm1 in living cells. Sqstm1–GFP was translocated to a restricted area of LC3-positive structures, which primarily correspond to the inside of autophagosomes, and then degraded. Moreover, exposure to arsenite induced expression of Sqstm1–GFP, followed by accumulation of the fusion protein in large aggregates that were degraded by autophagy. Furthermore, suppression of autophagy in Sqstm1-GFP^<KI/+> mouse livers caused accumulation of Sqstm1–GFP and formation of GFP-positive aggregate structures, leading to severe hepatic failure. These results indicate that Sqstm1-GFP^<KI/+> mice are a useful tool for analyzing Sqstm1 in living cells and intact animals.; 学位の種類: 博士（医学）. 報告番号: 甲第4127号. 学位記番号: 新大院博（医）甲第694号. 学位授与年月日: 平成28年3月23日; Journal of Cell Science. 2015, 128(23), 4453-4461.; 新大院博（医）甲第694号; 元資料の権利情報 : Copyright(C) 2015. Published by The Company of Biologists Ltd
• DOI: info:doi/10.1242/jcs.180174; https://doi.org/info:doi/10.1242/jcs.180174
• Persistent ID (NDL): info:ndljp/pid/10233206; https://dl.ndl.go.jp/pid/10233206
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-01-01T19:17:28+09:00
• Date Created (W3CDTF): 2019-08-05
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10191/41924
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2017-03-03 再収集