Corepressive function of nuclear receptor coactivator 2 in androgen receptor of prostate cancer cells treated with antiandrogen

• Material Type: 博士論文
• Title: Corepressive function of nuclear receptor coactivator 2 in androgen receptor of prostate cancer cells treated with antiandrogen
• Author/Editor: Takeda, Keisuke
• Publication, Distribution, etc.: 新潟大学
• Publication Date: 2016-09-20
• Publication Date (W3CDTF): 2016-09-20
• Alternative Title: Corepressive function of nuclear receptor coactivator 2 in androgen receptor of prostate cancer cells treated with antiandrogen; 前立腺癌細胞アンドロゲン受容体に対する抗アンドロゲン剤使用時のNCOA2の抑制共役因子作用
• Degree grantor/type: 新潟大学
• Date Granted: 2016-09-20
• Date Granted (W3CDTF): 2016-09-20
• Dissertation Number: 甲第4206号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第4206号
• Text Language Code: eng
• Subject Heading: Androgen receptor; Antiandrogen; Coactivator; Corepressor
• Note (General): Background: Recruitment of cofactors in the Interaction of the androgen receptor (AR) and AR ligands plays a critical role in determining androgenic/antiandrogenic effects of the AR ligand on signaling, but the functions of key cofactors, including nuclear receptor coactivator (NCOA), remain poorly understood in prostate cancer cells treated with AR ligands.\ Methods: We examined prostate cancer cell lines LNCaP and VCaP expressing mutated and wild-type ARs,respectively, to clarify the significance of NCOAs in the effect of antiandrogens. Hydroxyflutamide showed antagonistic activity against VCaP and an agonistic effect on LNCaP. Bicalutamide served as an antagonist for both.We analyzed mRNA transcription and protein expression of NCOAs in these cells pretreated with\ dihydrotestosterone and thereafter treated with the mentioned antiandrogens. Transcriptional silencing of candidate NCOAs and AR was performed using small interfering RNA (siRNA). Cell proliferation was evaluated with MTT assay.\ Results: LNCaP treated with bicalutamide showed an about four-fold increase in the expression of NCOA2 mRNA compared to those pretreated with dihydrotestosterone alone (P <0.01). In VCaP pretreated with dihydrotestosterone, transcriptions of NCOA2 and NCOA7 were slightly increased with bicalutamide (1.96- and 2.42-fold, respectively) and hydroxyflutamide (1.33-fold in both). With Western blotting, the expression of NCOA2 protein also increased in LNCaP cells treated with bicalutamide compared with that in control cells pretreated with dihydrotestosterone alone. Following silencing with siRNA for NCOA2, PSA levels in media with LNCaP receiving bicalutamide were elevated compared with those in non-silencing controls (101.6 ± 4.2 vs. 87.8 ± 1.4 ng/mL,respectively, P =0.0495). In LNCaP cells treated with dihydrotestosterone and bicalutamide, NCOA2-silencing was associated with a higher proliferation activity compared with non-silencing control and AR-silencing.Conclusion: NCOA2, which has been thought to be recruited as a coactivator, possibly plays a corepressive role in AR of prostate cancer cells when treated with antiandrogens, suggesting its potential as a therapeutic target.; 学位の種類: 博士（医学）. 報告番号: 甲第4206号. 学位記番号: 新大院博（医）甲第705号. 学位授与年月日: 平成28年9月20日; BMC Cancer(2016) 16:332; 新大院博（医）甲第705号
• DOI: info:doi/10.1186/s12885-016-2378-y; https://doi.org/info:doi/10.1186/s12885-016-2378-y
• Persistent ID (NDL): info:ndljp/pid/10233369; https://dl.ndl.go.jp/pid/10233369
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-01-01T19:17:28+09:00
• Date Created (W3CDTF): 2019-08-05
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://hdl.handle.net/10191/45092
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp