Efficacy of freeze-dried platelet-rich plasma in bone engineering

• Material Type: 博士論文
• ISSN: 0003-9969
• Title: Efficacy of freeze-dried platelet-rich plasma in bone engineering
• Volume: 73
• Author/Editor: 中谷, 佑哉
• Author Heading: 中谷, 佑哉
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2017-02-01
• Publication Date (W3CDTF): 2017-02-01
• Alternative Title: フリーズドライ保存した多血小板血漿は骨再生に有用である
• Periodical title: Archives of Oral Biology
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2017-02-01
• Date Granted (W3CDTF): 2017-02-01
• Dissertation Number: 甲医歯薬第913号
• Degree Type: 博士(歯学)
• Conferring No. (Dissertation): 甲医歯薬第913号
• Text Language Code: eng
• Subject Heading: Platelet-rich plasma; Freeze-Dry; Long storage; Super-concentrated platelets; Bone regeneration
• Target Audience: 一般
• Note (General): Objective: Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. Design: FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at ?20 °C for one month. It was then rehydrated with an equal or 1/3 amount of distilled water (×1FD-PRP, ×3FD-PRP, respectively), and we assessed its gelation properties and the release of growth factors (PDGF-BB, TGF-β1, and VEGF). We also examined the bone forming ability with onlay-grafting on mice calvaria using β-TCP granules as a scaffold. Results: FD-PRP showed comparable gelation as f-PRP. In terms of growth factor release, × 1FD-PRP released identical concentrations of PDGF-BB and TGF-β1 to f-PRP, while ×3FD-PRP released approximately 3-fold concentrations when compared with f-PRP. In vivo, ×1FD-PRP promoted identical levels of the bone formation as f-PRP, and ×3FD-PRP induced more abundant bone formation. Conclusions: These results suggest that f-PRP can be stored without functional loss by freeze-drying and the concentration of PRP may improve its efficacy in bone engineering.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第913号 学位授与年月日:平成29年2月1日; Author: Yuya Nakatani, Hideki Agata, Yoshinori Sumita, Takamitsu Koga, Izumi Asahina; Citation: Archives of Oral Biology, 73, pp.172-178; 2017; Nagasaki University (長崎大学), 博士(歯学) (2017-02-01)
• DOI: info:doi/10.1016/j.archoralbio.2016.10.006; https://doi.org/info:doi/10.1016/j.archoralbio.2016.10.006
• Persistent ID (NDL): info:ndljp/pid/10310783; https://dl.ndl.go.jp/pid/10310783
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-03-02T13:20:07+09:00
• Date Created (W3CDTF): 2020-12-21
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10069/37042; https://nagasaki-u.repo.nii.ac.jp/records/3857
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2021-03-07 再収集