Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes

• Material Type: 博士論文
• Title: Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes
• Author/Editor: フローレス, マリアジョリナルー; Flores, Maria Jolina Lou
• Author Heading: フローレス, マリアジョリナルー; Flores, Maria Jolina Lou
• Publication Date: 2014-06-01
• Publication Date (W3CDTF): 2014-06-01
• Alternative Title: ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究
• Degree grantor/type: 秋田大学
• Date Granted: 2014-03-22
• Date Granted (W3CDTF): 2014-03-22
• Dissertation Number: 甲第1088号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第1088号
• Text Language Code: eng
• Subject Heading: apical membrane; ceruloplasmin; GPI-Cp; hepatocyte
• Target Audience: 一般
• Note (General): Aim: Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositolanchored ceruloplasmin (GPI-Cp) form; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods: We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results: GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositolspecific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion: These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis.; http://hdl.handle.net/10295/2507
• Persistent ID (NDL): info:ndljp/pid/10318063; https://dl.ndl.go.jp/pid/10318063
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-04-02T16:10:31+09:00
• Date Created (W3CDTF): 2017-02-16
• Format (IMT): PDF
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• Related Material (URI): http://hdl.handle.net/10295/2507
• Periodical Title (URI): http://hdl.handle.net/10295/2509
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp