Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration.

• Material Type: 博士論文
• Title: Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration.
• Author/Editor: Sato, Ayako; Kajiya, Hiroshi; Mori, Nana; Sato, Hironobu; Fukushima, Tadao; Kido, Hirofumi; Ohno, Jun
• Author Heading: Sato, Ayako; Kajiya, Hiroshi; Mori, Nana; Sato, Hironobu; Fukushima, Tadao; Kido, Hirofumi; Ohno, Jun
• Publication Date: 2017-03-11
• Publication Date (W3CDTF): 2017-03-11
• Alternative Title: Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2017-03-11
• Date Granted (W3CDTF): 2017-03-11
• Dissertation Number: 甲第287号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第287号
• Text Language Code: eng
• Subject Heading: DNA; 骨再生; 骨分化; 頭蓋骨欠損; 細胞遊走
• Target Audience: 一般
• Note (General): The initial step of bone regeneration requires the migration of osteogenic cells to defective sites. Our previous studies suggest that a salmon DNA-based scaffold can promote the bone regeneration of calvarial defects in rats. We speculate that the salmon DNA may possess osteoinductive properties, including the homing of migrating osteogenic cells. In the present study, we investigated the influence of the salmon DNA on osteoblastic differentiation and induction of osteoblast migration using MG63 cells (human preosteoblasts) in vitro. Moreover, we analyzed the bone regeneration of a critical-sized in vivo calvarial bone defect (CSD) model in rats. The salmon DNA enhanced both mRNA and protein expression of the osteogenesis-related factors, runt-related transcription factor 2 (Runx2), alkaline phosphatase, and osterix (OSX) in the MG63 cells, compared with the cultivation using osteogenic induction medium alone. From the histochemical and immunohistochemical assays using frozen sections of the bone defects from animals that were implanted with DNA disks, many cells were found to express aldehyde dehydrogenase 1, one of the markers for mesenchymal stem cells. In addition, OSX was observed in the replaced connective tissue of the bone defects. These findings indicate that the DNA induced the migration and accumulation of osteogenic cells to the regenerative tissue. Furthermore, an in vitro transwell migration assay showed that the addition of DNA enhanced an induction of osteoblast migration, compared with the medium alone. The implantation of the DNA disks promoted bone regeneration in the CSD of rats, compared with that of collagen disks. These results indicate that the salmon DNA enhanced osteoblastic differentiation and induction of migration, resulting in the facilitation of bone regeneration.; PLOS ONE
• Persistent ID (NDL): info:ndljp/pid/10318545; https://dl.ndl.go.jp/pid/10318545
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-04-02T16:10:31+09:00
• Date Created (W3CDTF): 2017-03-15
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169522
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/69
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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