Roles of hypoxia inducible factor-1α in the temporomandibular joint

• Material Type: 博士論文
• ISSN: 0003-9969
• Title: Roles of hypoxia inducible factor-1α in the temporomandibular joint
• Volume: 73
• Author/Editor: Mino-Oka, Akiko; Izawa, Takashi; Shinohara, Takehiro; Mori, Hiroki; Yasue, Akihiro; Tomita, Shuhei; Tanaka, Eiji
• Author Heading: Mino-Oka, Akiko; Izawa, Takashi; Shinohara, Takehiro; Mori, Hiroki; Yasue, Akihiro; Tomita, Shuhei; Tanaka, Eiji
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2016-10-28
• Publication Date (W3CDTF): 2016-10-28
• Alternative Title: 顎関節におけるHIF-1αの役割
• Periodical title: Archives of Oral Biology
• Degree grantor/type: 徳島大学
• Date Granted: 2017-03-23
• Date Granted (W3CDTF): 2017-03-23
• Dissertation Number: 甲第3072号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第3072号
• Text Language Code: eng
• Subject Heading: TMJ-OA; HIF-1α; Articular cartilage degradation; chondrocyte apoptosis
• Target Audience: 一般
• Note (General): Objective: Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease characterized by permanent cartilage loss. Articular cartilage is maintained in a low-oxygen environment. The chondrocyte response to hypoxic conditions involves expression of hypoxia inducible factor 1α (HIF-1α), which induces chondrocytes to increase expression of vascular endothelial growth factor (VEGF). Here, we investigated the role of HIF-1α in mechanical load effects on condylar cartilage and subchondral bone in heterozygous HIF-1α-deficient mice (HIF-1α+/-).Design: Mechanical stress was applied to the TMJ of C57BL/6NCr wild-type (WT) and HIF-1α+/- mice with a sliding plate for 10 days. Histological analysis was performed by HE staining, Safranin-O/Fast green staining, and immunostaining specific for articular cartilage homeostasis.Results: HIF-1α+/- mice had thinner cartilage and smaller areas of proteoglycan than WT controls, without and with mechanical stress. Mechanical stress resulted in prominent degenerative changes with increased expression of HIF-1α, VEGF, and the apoptosis factor cleaved Caspase-3 in condylar cartilage.Conclusion: Our results indicate that HIF-1a may be important for articular cartilage homeostasis and protective against articular cartilage degradation in the TMJ under mechanical stress condition, therefore HIF-1α could be an important new therapeutic target in TMJ-OA.
• DOI: info:doi/10.1016/j.archoralbio.2016.10.028; https://doi.org/info:doi/10.1016/j.archoralbio.2016.10.028
• Persistent ID (NDL): info:ndljp/pid/10370749; https://dl.ndl.go.jp/pid/10370749
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-07-03T04:10:06+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/110094
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp