Functional characterization of tissue factor in von Willebrand factor-dependent thrombus formation under whole blood flow conditions.

• Material Type: 博士論文
• ISSN: 0925-5710
• Title: Functional characterization of tissue factor in von Willebrand factor-dependent thrombus formation under whole blood flow conditions.
• Volume: 104 6
• Author/Editor: Matsunari, Yasunori; Sugimoto, Mitsuhiko; Doi, Masaaki; Matsui, Hideto; Kawaguchi, Masahiko
• Author Heading: Matsunari, Yasunori; Sugimoto, Mitsuhiko; Doi, Masaaki; Matsui, Hideto; Kawaguchi, Masahiko
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2016-12; 104 6
• Publication Date (W3CDTF): 2016-12
• Alternative Title: 全血流動下でのフォンビルブランド因子依存性血栓形成における組織因子の機能特性
• Periodical title: International journal of hematology
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2016-11-24
• Date Granted (W3CDTF): 2016-11-24
• Dissertation Number: 乙第1388号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第1388号
• Text Language Code: eng
• Subject Heading: Blood flow; Fibrin; Thrombus formation; Tissue factor; Von Willebrand factor
• Target Audience: 一般
• Note (General): type:Thesis; Von Willebrand factor (VWF) plays an important role in mediating platelet adhesion and aggregation under high shear rate conditions. Such platelet aggregates are strengthened by fibrin-network formation triggered by tissue factor (TF). However, little is known about the role of TF in VWF-dependent thrombus formation under blood flow conditions. We evaluated TF in thrombus formation on immobilized VWF under whole blood flow conditions in an in vitro perfusion chamber system. Surface-immobilized TF amplified intra-thrombus fibrin generation significantly under both low and high shear flow conditions, while TF in sample blood showed no appreciable effects. Furthermore, immobilized TF enhanced VWF-dependent platelet adhesion and aggregation significantly under high shear rates. Neutrophil cathepsin G and elastase increased significantly intra-thrombus fibrin deposition on immobilized VWF–TF complex, suggesting the involvement of leukocyte inflammatory responses in VWF/TF-dependent mural thrombogenesis under these flow conditions. These results reveal a functional link between VWF and TF under whole blood flow conditions, in which surface-immobilized TF and VWF mutually contribute to mural thrombus formation, which is essential for normal hemostasis. By contrast, TF circulating in blood may be involved in systemic hypercoagulability, as seen in sepsis caused by severe microbial infection, in which neutrophil inflammatory responses may be active.; 博士（医学）・乙第1388号・平成28年11月24日; © The Japanese Society of Hematology 2016; The original publication is available at www.springerlink.com; The final publication is available at Springer via http://dx.doi.org/10.1007/s12185-016-2086-z; identifier:International journal of hematology Vol.104 No.6 p.661-668 (2016 Dec); identifier:09255710; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3293; identifier:International journal of hematology, 104(6): 661-668
• DOI: info:doi/10.1007/s12185-016-2086-z; https://doi.org/info:doi/10.1007/s12185-016-2086-z
• Persistent ID (NDL): info:ndljp/pid/10402668; https://dl.ndl.go.jp/pid/10402668
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-08-02T04:31:34+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10564/3293
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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