Nanogel-Based Immunologically Stealth Vaccine Targets Macrophages in the Medulla of Lymph Node and Induces Potent Antitumor Immunity

• Material Type: 博士論文
• Title: Nanogel-Based Immunologically Stealth Vaccine Targets Macrophages in the Medulla of Lymph Node and Induces Potent Antitumor Immunity
• Author/Editor: Muraoka, Daisuke
• Author Heading: Muraoka, Daisuke
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2016-03-25
• Publication Date (W3CDTF): 2016-03-25
• Degree grantor/type: 三重大学
• Date Granted: 2016-03-25
• Date Granted (W3CDTF): 2016-03-25
• Dissertation Number: 甲医学第1757号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1757号
• Text Language Code: eng
• Alias of Author: ムラオカ, ダイスケ; 村岡, 大輔
• Subject Heading: cancer vaccine; nanogel; vaccine delivery; macrophages; lymph node; T cells
• Target Audience: 一般
• Note (General): application/pdf; Because existing therapeutic cancer vaccines provide only a limited clinical benefit, a different vaccination strategy is necessary to improve vaccine efficacy. We developed a nanoparticulate cancer vaccine by encapsulating a synthetic long peptide antigen within an immunologically inert nanoparticulate hydrogel (nanogel) of cholesteryl pullulan (CHP). After subcutaneous injection to mice, the nanogel-based vaccine was efficiently transported to the draining lymph node, and was preferentially engulfed by medullary macrophages but was not sensed by other macrophages and dendritic cells (so-called “immunologically stealth mode”). Although the function of medullary macrophages in T cell immunity has been unexplored so far, these macrophages effectively cross-primed the vaccine-specific CD8＋ T cells in the presence of a Toll-like receptor (TLR) agonist as an adjuvant. The nanogelbased vaccine significantly inhibited in vivo tumor growth in the prophylactic and therapeutic settings, compared to another vaccine formulation using a conventional delivery system, incomplete Freund's adjuvant. We also revealed that lymph node macrophages were highly responsive to TLR stimulation, which may underlie the potency of the macrophage-oriented, nanogel-based vaccine. These results indicate that targeting medullary macrophages using the immunologically stealth nanoparticulate delivery system is an effective vaccine strategy.; 本文 / Department of Immuno-Gene Therapy, Mie University Graduate School of Medicine; 10p; http://pubs.acs.org/doi/abs/10.1021/nn502975r
• DOI: info:doi/10.1021/nn502975r; https://doi.org/info:doi/10.1021/nn502975r
• Persistent ID (NDL): info:ndljp/pid/10402937; https://dl.ndl.go.jp/pid/10402937
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-08-02T04:31:34+09:00
• Date Created (W3CDTF): 2017-07-04
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://pubs.acs.org/doi/abs/10.1021/nn502975r
• Periodical Title (URI): http://hdl.handle.net/10076/00016967; https://mie-u.repo.nii.ac.jp/records/11311
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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