Significant intratumoral heterogeneity of HER2 status in gastric cancer: a comparative study among IHC, FISH, and DISH

• Material Type: 博士論文
• Title: Significant intratumoral heterogeneity of HER2 status in gastric cancer: a comparative study among IHC, FISH, and DISH
• Author/Editor: Kanayama, Kazuki
• Author Heading: Kanayama, Kazuki
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2016-03-25
• Publication Date (W3CDTF): 2016-03-25
• Degree grantor/type: 三重大学
• Date Granted: 2016-03-25
• Date Granted (W3CDTF): 2016-03-25
• Dissertation Number: 甲医学第1761号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1761号
• Text Language Code: eng
• Alias of Author: カナヤマ, カズキ; 金山, 和樹
• Subject Heading: Human epidermal growth factor receptor 2; intratumoral heterogeneity; biopsy specimens; dual-color in situ hybridization; gastric cancer
• Target Audience: 一般
• Note (General): application/pdf; The assessment of human epidermal growth factor receptor 2 (HER2) status is crucial for selecting with patients with gastric cancer who may benefit from HER2-targeted therapy. Accurate assessment using biopsy specimens is important for patients with advanced-stage cancer. Intratumoral heterogeneity of HER2, however, is a major challenge in HER2 testing. Here, we aimed to examine whether assessment of HER2 status could be accurately performed with currently used methods, i.e., immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and dual-color in situ hybridization (DISH). HER2 status was evaluated in 108 biopsy tissues from patients with gastric adenocarcinoma and 70 matched surgical specimens by IHC, FISH, and DISH. HER2 amplification was detected in 11 (10.2%) out of 108 biopsy specimens. IHC and FISH . IHC and FISH . IHC and FISH . IHC and FISH . IHC and FISH . IHC and FISH . IHC and FISH . IHC and FISH . IHC and FISH results were well correlated, and FISH and DISH results were consistent for all cases. The overall concordance rate of HERS status between biopsy tissues and surgical specimens was 91.4%. All six discordant cases were false negative on biopsy; of these cases, five showed HER2 heterogeneity on surgical resection. Assessment of the HER2 status of biopsy tissues could predict the status of the whole tumor; however a proportion of these cases may be discordant because of intratumoral heterogeneity.; 本文 / Department of Oncologic Pathology, Mie University Graduate School of Medicine; 26p; http://onlinelibrary.wiley.com/doi/10.1111/cas.12886/full
• DOI: info:doi/10.1111/cas.12886; https://doi.org/info:doi/10.1111/cas.12886
• Persistent ID (NDL): info:ndljp/pid/10402939; https://dl.ndl.go.jp/pid/10402939
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-08-02T04:31:34+09:00
• Date Created (W3CDTF): 2017-07-04
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://onlinelibrary.wiley.com/doi/10.1111/cas.12886/full
• Periodical Title (URI): http://hdl.handle.net/10076/00016969; https://mie-u.repo.nii.ac.jp/records/11313
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2017-11-03 再収集

2023-12-15 再収集