Regulatory mechanism of motilin-induced gastrointestinal motility in Japanese quail

• Material Type: 博士論文
• Title: Regulatory mechanism of motilin-induced gastrointestinal motility in Japanese quail
• Author/Editor: AUVIJIT, SAHA APU
• Author Heading: AUVIJIT, SAHA APU
• Publication, Distribution, etc.: 埼玉大学大学院理工学研究科
• Publication Date: 2016
• Publication Date (W3CDTF): 2016
• Alternative Title: ウズラにおけるモチリン誘導性消化管運動調節機構の研究
• Periodical title: 博士論文（埼玉大学大学院理工学研究科（博士後期課程））
• Degree grantor/type: 埼玉大学
• Date Granted: 2016-09-23
• Date Granted (W3CDTF): 2016-09-23
• Dissertation Number: 甲第1024号
• Degree Type: 博士(学術)
• Conferring No. (Dissertation): 甲第1024号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): type:text; Motilin is a peptide hormone that plays an important role in the regulation of gastrointestinal (GI) motility. In the current study, we determined the cDNA and amino acid sequences of motilin and its receptor (GPR38) in Japanese quail, and studied the distribution of motilin-producing cells and the expression of GPR38 mRNA in the quail GI tract. Using an in vitro organ bath, we examined the motilin-induced contractile properties of different regions of the quail GI tract. We also determined the age-dependent changes and mechanisms of motilin-induced contraction in the proventriculus and duodenum of the quail. Mature quail motilin was composed of 22 amino acids, and showed high homology to chicken (95.4%), human (72.7%), and dog (72.7%) motilin. Immunohistochemical analysis revealed that motilin-immunopositive cells were present in the mucosal layer of the duodenum (23.4 ± 4.6 cells/mm2), jejunum (15.2 ± 0.8 cells/mm2), and ileum (2.5 ± 0.7 cells/mm2), but were not observed in the crop, proventriculus, and colon. We also cloned quail GPR38-encoding cDNA from the medulla oblongata and obtained a partial sequence of 183 bp. Real-time PCR analysis showed that quail GPR38 mRNA was expressed in the crop, proventriculus, duodenum, jejunum, ileum, colon, liver, heart, and muscle. In the organ bath study, chicken motilin evoked dose-dependent contraction of the proventriculus, duodenum, jejunum, and ileum. In contrast, chicken ghrelin had no effect on contraction in the quail GI tract. Motilin-induced contraction in the duodenum was not inhibited by atropine, hexamethonium, ritanserin, ondansetron, GR125487, or tetrodotoxin. However, motilin-induced contractions in the proventriculus were significantly inhibited by atropine and tetrodotoxin. In addition, motilin-induced contraction was significantly decreased with age in the proventriculus, but not in the duodenum. These results suggest that motilin stimulates gastrointestinal motility, and that the site of action for motilin is different between the small intestine and proventriculus of Japanese quail.; ABSTRACT ITABLE OF CONTENTS IIILIST OF FIGURES VILIST OF APPENDICES VIIIChapter 1: General introduction and objectives 11.1. Research background 11.1.1. The migrating motor complex of gastrointestinal (GI) motility 11.1.2. Motilin 21.1.3. Ghrelin 31.1.4. Co-ordinated action of motilin and ghrelin in the GI tract 51.1.5. Regulatory mechanism of motilin-induced GI contraction 51.1.6. Japanese quail (Coturnix japonica) 61.2. Purpose of the study 8Chapter 2: Molecular cloning of motilin and mechanism of motilin-induced gastrointestinal motility in Japanese quail 92.1. Introduction 92.2. Materials and Methods 122.2.1. Drugs used 122.2.2. Animals 122.2.3. Cloning of quail motilin cDNA 122.2.4. Tissue preparation 132.2.4.1. Immunohistochemistry 142.2.4.2. Morphometric analysis 152.2.5. Organ bath study 152.2.6. Statistical analysis 172.3. Results 182.3.1. Cloning of quail motilin cDNA 182.3.2. Localization of motilin immunopositive (motilin-ip) cells in the GI tract of quail 182.3.3. Effects of motilin on the GI tract of quail 192.3.4. Effects of ghrelin on the GI tract of quail 192.3.5. Effects of co-administration of motilin and ghrelin on the GI tract of quail 202.3.6. Mechanism of chicken motilin-induced contraction in the duodenum of quail 202.3.7. Mechanism of chicken motilin-induced contraction in the proventriculus of quail 202.4. Discussion 222.4.1. cDNA and amino acid sequences of quail motilin 222.4.2. Immunohistochemical analysis of quail motilin 222.4.3. Effects of motilin and ghrelin, alone or together, on gastrointestinal motility in Japanese quail 232.4.4. Mechanism of motilin-induced contraction in quail proventriculus and duodenum 232.4.5. Physiological significance of MMC in relation to motilin 252.5. Summary 26Chapter 3: Molecular identification and expression of GPR38 in the gastrointestinal tract of Japanese quail 273.1. Introduction 273.2. Materials and methods 303.2.1. Animals 303.2.2. Cloning of quail GPR38 303.2.3. Reverse transcription (RT) and quantitative real-time PCR (Q-PCR) analysis 313.2.4. Organ bath study 323.2.5. Statistical analysis 333.3. Results 343.3.1. Cloning of quail GPR38 343.3.2. Expression of GPR38 mRNA in quail tissues 343.3.3. Age-dependent changes in motilin-induced contraction in the GI tract of Japanese quail 343.4. Discussion 363.5. Summary 394.0. CONCLUSION 40ABBREVIATIONS 41REFERENCES 43FIGURES 63APPENDICES 87ACKNOWLEDGEMENTS 92; 主指導教員 : 坂田一郎
• DOI: 10.24561/00010414; https://doi.org/10.24561/00010414
• Persistent ID (NDL): info:ndljp/pid/10403310; https://dl.ndl.go.jp/pid/10403310
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• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-08-02T04:31:34+09:00
• Date Created (W3CDTF): 2017-07-19
• Format (IMT): application/pdf
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• Periodical Title (URI): http://dx.doi.org/10.24561/00010414; http://id.nii.ac.jp/1586/00010414/
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-09-04 再収集