Beneficial effect of D-allose for isolated islet culture prior to islet transplantation

Persistent ID (NDL): info:ndljp/pid/10958783

Material type: 博士論文

Author: 柏木, 裕貴

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Publication date: 2017-03-24

Material Format: Digital

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Name of awarding university/degree: 香川大学,博士（医学）

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Note (General)：: BackgroundPretransplant restoration of islets damaged during isolation remains to be solved. In this study, we examined the effect of D‐allose on isle...

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Material Type: 博士論文
Title: Beneficial effect of D-allose for isolated islet culture prior to islet transplantation
Author/Editor: 柏木, 裕貴
Author Heading: 柏木, 裕貴
Publication Date: 2017-03-24
Publication Date (W3CDTF): 2017-03-24
Degree grantor/type: 香川大学
Date Granted: 2017-03-24
Date Granted (W3CDTF): 2017-03-24
Dissertation Number: 甲第666号
Degree Type: 博士（医学）
Conferring No. (Dissertation): 甲第666号
Text Language Code: eng
Alias of Author: Kashiwagi, Hirotaka
Subject Heading: D-allose
Islet transplantation
Rare sugar
Target Audience: 一般
Note (General): BackgroundPretransplant restoration of islets damaged during isolation remains to be solved. In this study, we examined the effect of D‐allose on islets isolated from rat pancreata prior to islet transplantation.
MethodsRat islets isolated from fresh pancreata were cultured overnight in Roswell Park Memorial Institute 1640 solution in the absence (group 1) or presence (group 2) of D‐allose. Then we assessed stimulation index of insulin, and cure rate after islet transplantation to diabetic nude mice. We also measured malondialdehyde level and caspase 3 activity of islets after the overnight culture for assessment of the oxidative stress and the apoptosis.
ResultsD‐allose significantly improved insulin secretion of islets. The stimulation index in group 2 was significantly higher than in group 1. Cure rate after transplantation in group 2 was higher than in group 1 especially in the first week. The malondialdehyde level in group 2 was significantly lower than in group 1. But the caspase 3 activities in both groups did not differ.
ConclusionsD‐allose treatment of isolated islet culture prior to transplantation restored islet function and increased successful transplant rate. The results of this study suggested that D‐allose improved function of damaged islets through its anti‐oxidative activity.
DOI: info:doi/10.1002/jhbp.298
https://doi.org/info:doi/10.1002/jhbp.298
Persistent ID (NDL): info:ndljp/pid/10958783
https://dl.ndl.go.jp/pid/10958783
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2017-10-02T17:34:20+09:00
Date Created (W3CDTF): 2020-10-27
Format (IMT): PDF
Access Restrictions: 国立国会図書館内限定公開
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Periodical Title (URI): https://kagawa-u.repo.nii.ac.jp/records/286
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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