Campylobacter jejuni infection suppressed Cl⁻ secretion induced by CFTR activation in T-84 cells

• Material Type: 博士論文
• ISSN: 1341-321X
• Title: Campylobacter jejuni infection suppressed Cl⁻ secretion induced by CFTR activation in T-84 cells
• Volume: 20 11
• Author/Editor: Negoro, Sachie; Shimohata, Takaaki; Hatayama, Syo; Sato, Yuri; Matsumoto, Mari; Iba, Hitomi; Aihara, Mutsumi; Uebansou, Takashi; Hamada, Yasuhiro; Nishikawa, Yoshikazu; Yamasaki, Shinji; Mawatari, Kazuaki; Takahashi, Akira
• Author Heading: Negoro, Sachie; Shimohata, Takaaki; Hatayama, Syo; Sato, Yuri; Matsumoto, Mari; Iba, Hitomi; Aihara, Mutsumi; Uebansou, Takashi; Hamada, Yasuhiro; Nishikawa, Yoshikazu; Yamasaki, Shinji; Mawatari, Kazuaki; Takahashi, Akira
• Publication, Distribution, etc.: Japanese Society of Chemotherapy and The Japanese Association for Infection Disease|Elsevier
• Publication Date: 2014-08-06
• Publication Date (W3CDTF): 2014-08-06
• Alternative Title: Campylobacter jejuni感染はT-84細胞におけるCFTR活性化によるCl⁻分泌亢進を抑制する
• Periodical title: Journal of Infection and Chemotherapy
• Degree grantor/type: 徳島大学
• Date Granted: 2017-03-23
• Date Granted (W3CDTF): 2017-03-23
• Dissertation Number: 甲第3089号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3089号
• Text Language Code: eng
• Subject Heading: Campylobacter jejuni; CFTR; diarrhea; short-circuit current; efflux assay
• Target Audience: 一般
• Note (General): Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl-) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl- channel transporting Cl- to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied.Here, we used an 125I- efflux assay and measurement of short-circuit current to measure Cl- secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl- secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl- secretion suppressed in C. jejuniinfected T-84 cells. The suppression of activated Cl- secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract.
• DOI: info:doi/10.1016/j.jiac.2014.07.007; https://doi.org/info:doi/10.1016/j.jiac.2014.07.007
• Persistent ID (NDL): info:ndljp/pid/10993570; https://dl.ndl.go.jp/pid/10993570
• Collection: 障害者向け資料
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-12-04T02:02:48+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/110108
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp