Antibacterial and Antibiofilm Effects of Reuterin-Related Compounds to Periodontopathic Bacteria

• Material Type: 博士論文
• ISSN: 2189-6682
• Title: Antibacterial and Antibiofilm Effects of Reuterin-Related Compounds to Periodontopathic Bacteria
• Volume: 30 1
• Author/Editor: Fujiwara, Natsumi; Murakami, Keiji; Nakao, Michiyasu; Toguchi, Munehisa; Yumoto, Hiromichi; Hirota, Katsuhiko; Matsuo, Takashi; Sano, Shigeki; Ozaki, Kazumi; Miyake, Yoichiro
• Author Heading: Fujiwara, Natsumi; Murakami, Keiji; Nakao, Michiyasu; Toguchi, Munehisa; Yumoto, Hiromichi; Hirota, Katsuhiko; Matsuo, Takashi; Sano, Shigeki; Ozaki, Kazumi; Miyake, Yoichiro
• Publication, Distribution, etc.: 四国歯学会
• Publication Date: 2017-06-30
• Publication Date (W3CDTF): 2017-06-30
• Alternative Title: ロイテリン関連化合物の歯周病原因菌への抗菌および抗バイオフィルム効果
• Periodical title: Journal of Oral Health and Biosciences
• Degree grantor/type: 徳島大学
• Date Granted: 2017-03-09
• Date Granted (W3CDTF): 2017-03-09
• Dissertation Number: 甲第3076号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第3076号
• Text Language Code: eng
• Subject Heading: Lactobacillus reuteri; reuterin; Fusobacterium nucleatum; biofilm
• Target Audience: 一般
• Note (General): OBJECTIVE: Lactobacillus reuteri is one of the probiotics that possesses preventive effects on oral infections including dental caries and periodontal disease. Reuterin is a bacteriocin-like compound produced by L. reuteri and plays an important role in the probiotic effects. However, it is difficult to obtain reuterin due to the general lability of its aldehyde moiety. Therefore, fourteen stable reuterin-related compounds (RRCs) were chemically synthesized. Here, we investigated the effects of RRCs on periodontopathic bacteria.MATERIALS AND METHODS: The antibacterial activity of RRCs on pathogenic microorganisms including periodontopathic bacteria was determined. Moreover, the effects of RRCs on biofilm formation by Fusobacterium nucleatum were examined by a crystal violet biofilm formation assay. Cytotoxicity of RRCs was evaluated by a lactate dehydrogenase assay using oral keratinocytes.RESULTS: Among synthesized RRCs, RRC-04, -05, -09, -10, -12, -13, and -14, exhibited antibacterial activities against periodontopathic bacteria. RRCs, except for RRC-06, -07, -08, and -11, significantly suppressed biofilm formation to 60-80 % of the control. Most RRCs, except for RRC-12 and -13, were not cytotoxic to human oral keratinocytes.CONCLUSIONS: The synthesized RRCs can be potent novel oral care products for the prevention of periodontitis without adverse effects.
• DOI: info:doi/10.20738/johb.30.1_8; https://doi.org/info:doi/10.20738/johb.30.1_8
• Persistent ID (NDL): info:ndljp/pid/10993574; https://dl.ndl.go.jp/pid/10993574
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-12-04T02:02:48+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): https://repo.lib.tokushima-u.ac.jp/111063
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp