Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation

• Material Type: 博士論文
• Title: Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation
• Author/Editor: Furukawa, Kazuo
• Publication, Distribution, etc.: 新潟大学
• Publication Date: 2017-09-20
• Publication Date (W3CDTF): 2017-09-20
• Alternative Title: Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation; 最終糖化産物(AGEs)はヒト血小板において、Srcファミリーキナーゼの活性化を介して脳由来神経栄養因子(BDNF)の放出を促進させる
• Degree grantor/type: 新潟大学
• Date Granted: 2017-09-20
• Date Granted (W3CDTF): 2017-09-20
• Dissertation Number: 甲第4357号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第4357号
• Text Language Code: eng
• Subject Heading: AGE; BDNF; Platelets; Release; Ca^<2+>; Src family kinases; Syk
• Note (General): Background: Brain-derived neurotrophic factor (BDNF) exerts beneficial effects not only on diabetic neuropathies but also on cardiovascular injury. There is argument regarding the levels of serum BDNF in patients with diabetes mellitus (DM). Because BDNF in peripheral blood is rich in platelets, this may represent dysregulation of BDNF release from platelets. Here we focused on advanced glycation end products (AGEs), which are elevated in patients with DM and have adverse effects on cardiovascular functions. The aim of this study is to elucidate the role of AGEs in the regulation of BDNF release from human platelets. Methods: Platelets collected from peripheral blood of healthy volunteers were incubated with various concentrations of AGE (glycated-BSA) at 37 °C for 5 min with or without BAPTA-AM, a cell permeable Ca^<2+> chelator, or PP2, a potent inhibitor of Src family kinases (SFKs). Released and cellular BDNF were measured by ELISA and calculated. Phosphorylation of Src and Syk, a downstream kinase of SFKs, in stimulated platelets was examined by Western blotting and immunoprecipitation. Results: AGE induced BDNF release from human platelets in a dose-dependent manner, which was dependent on intracellular Ca^<2+> and SFKs. We found that AGE induced phosphorylation of Src and Syk. Conclusions: AGE induces BDNF release from human platelets through the activation of the Src-Syk-(possibly phospholipase C)-Ca^<2+> pathway. Considering the toxic action of AGEs and the protective roles of BDNF, it can be hypothesized that AGE-induced BDNF release is a biological defense system in the early phase of diabetes. Chronic elevation of AGEs may induce depletion or downregulation of BDNF in platelets during the progression of DM.; 学位の種類: 博士（医学）. 報告番号: 甲第4357号. 学位記番号: 新大院博（医）甲第770号. 学位授与年月日: 平成29年9月20日; Cardiovascular Diabetology. 2017, 16, 20.; 新大院博（医）甲第770号; 元資料の権利情報 : (C) The Author(s) 2017
• DOI: info:doi/10.1186/s12933-017-0505-y; https://doi.org/info:doi/10.1186/s12933-017-0505-y
• Persistent ID (NDL): info:ndljp/pid/10993759; https://dl.ndl.go.jp/pid/10993759
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-12-04T02:02:48+09:00
• Date Created (W3CDTF): 2019-08-05
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://hdl.handle.net/10191/48151
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp