Identification of cellular genes showing differential expression associated with hepatitis B virus infection

• Material Type: 博士論文
• Title: Identification of cellular genes showing differential expression associated with hepatitis B virus infection
• Author/Editor: Fukuhara, Yasuo
• Publication, Distribution, etc.: 新潟大学
• Publication Date: 2017-09-20
• Publication Date (W3CDTF): 2017-09-20
• Alternative Title: Identification of cellular genes showing differential expression associated with hepatitis B virus infection; HBV感染に伴い特異的に発現変動する宿主遺伝子の同定
• Degree grantor/type: 新潟大学
• Date Granted: 2017-09-20
• Date Granted (W3CDTF): 2017-09-20
• Dissertation Number: 乙第2213号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第2213号
• Text Language Code: eng
• Subject Heading: Hepatitis B virus; Differential gene expression; Hepatocellular carcinoma; Gene expression signature; Adipose most abundant 2
• Note (General): AIM: To investigate the impact of hepatitis B virus (HBV) infection on cellular gene expression, by conducting both in vitro and in vivo studies. METHODS: Knockdown of HBV was targeted by stable expression of short hairpin RNA (shRNA) in huH-1 cells. Cellular gene expression was compared using a human 30K cDNA microarray in the cells and quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR) (qRT-PCR) in the cells, hepatocellular carcinoma (HCC) and surrounding non-cancerous liver tissues (SL). RESULTS: The expressions of HBsAg and HBx protein were markedly suppressed in the cells and in HBx transgenic mouse liver, respectively, after introduction of shRNA. Of the 30K genes studied, 135 and 103 genes were identified as being down- and up-regulated, respectively, by at least twofold in the knockdown cells. Functional annotation revealed that 85 and 62 genes were classified into four up-regulated and five downregulated functional categories, respectively. When gene expression levels were compared between HCC and SL, eight candidate genes that were confirmed to be up- or down-regulated in the knockdown cells by both microarray and qRT-PCR analyses were not expressed as expected from HBV reduction in HCC, but had similar expression patterns in HBV- and hepatitis C virus-associated cases. In contrast, among the eight genes, only APM2 was constantly repressed in HBV non-associated tissues irrespective of HCC or SL. CONCLUSION: The signature of cellular gene expression should provide new information regarding the pathophysiological mechanisms of persistent hepatitis and hepatocarcinogenesis that are associated with HBV infection.; 学位の種類: 博士（医学）. 報告番号: 乙第2213号. 学位記番号: 新大博（医）乙第1788号. 学位授与年月日: 平成29年9月20日; World Journal of Hepatology. 2012, 4(4), 139-148.; 新大博（医）乙第1788号; 元資料の権利情報 : (C) 2012 Baishideng. All rights reserved.
• DOI: info:doi/10.4254/wjh.v4.i4.139; https://doi.org/info:doi/10.4254/wjh.v4.i4.139
• Persistent ID (NDL): info:ndljp/pid/10993768; https://dl.ndl.go.jp/pid/10993768
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-12-04T02:02:48+09:00
• Date Created (W3CDTF): 2019-08-05
• Format (IMT): application/pdf
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• Periodical Title (URI): http://hdl.handle.net/10191/48162
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp