Tumor necrosis factor-α antagonist suppresses local inflammatory reaction and facilitates olfactory nerve recovery following injury

• Material Type: 博士論文
• Title: Tumor necrosis factor-α antagonist suppresses local inflammatory reaction and facilitates olfactory nerve recovery following injury
• Author/Editor: Al, Salihi Mohammed Omar
• Author Heading: Al, Salihi Mohammed Omar
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2016-09-21
• Publication Date (W3CDTF): 2016-09-21
• Degree grantor/type: 三重大学
• Date Granted: 2016-09-21
• Date Granted (W3CDTF): 2016-09-21
• Dissertation Number: 甲医学第1797号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1797号
• Text Language Code: eng
• Subject Heading: Cycloheximide; Etanercept; Field potential recording; Olfactory nerve; Regeneration; Tumor necrosis factor-alpha (TNF-α)
• Target Audience: 一般
• Note (General): application/pdf; Objective: Olfactory dysfunction is a common finding in head trauma due to injury to the olfactory nerve. We previously reported that anti-inflammatory treatment with steroids improves recovery outcome in olfactory nerve injury models. Clinically, however, steroid administration is not recommended in the acute phase of head injury cases because of concerns regarding its side effects. Tumor necrosis factor (TNF-α) is known to play a key role in inflammatory response to injury. The present study examines if the inhibition of TNF-α can facilitate functional recovery in the olfactory system following injury. Materials and methods: Olfactory nerve transection (NTx) was performed in olfactory marker protein (OMP-tau-lacZ) mice to establish injury models. We measured TNF-α gene expression in the olfactory bulb using semi-quantitative and real time polymerase chain reaction (PCR) assays and found that they increase within hours after NTx injury. A TNF-α antagonist (etanercept) was intraperitoneally injected immediately after the NTx and histological assessment of recovery within the olfactory bulb was performed at 5–70 days. X-gal staining labeled OMP in the degenerating and regenerating olfactory nerve fibers, and immunohistochemical staining detected the presence of reactive astrocytes and macrophages/microglia. Results: Etanercept-injected mice showed significantly smaller areas of injury-associated tissue, fewer astrocytes and macrophages/microglia, and an increase in regenerating nerve fibers. Olfactory function assessments using both an olfactory avoidance behavioral test and evoked potential recordings showed improved functional recovery in etanercept-injected animals. Conclusion: These findings suggest that inhibition of TNF-α could provide a new therapeutic strategy for the treatment of olfactory dysfunction following head injuries.; 本文 / Department of Otorhinolaryngology-Head and Neck Surgery, Mie University Graduate School of Medicine; 9p
• DOI: info:doi/10.1016/j.anl.2016.05.009; https://doi.org/info:doi/10.1016/j.anl.2016.05.009
• Persistent ID (NDL): info:ndljp/pid/10993785; https://dl.ndl.go.jp/pid/10993785
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-12-04T02:02:48+09:00
• Date Created (W3CDTF): 2017-11-07
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10076/00017106; https://mie-u.repo.nii.ac.jp/records/11462
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2023-12-15 再収集