LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin

• Material Type: 博士論文
• Title: LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin
• Author/Editor: Kawamoto, Eiji
• Author Heading: Kawamoto, Eiji
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2017-03-24
• Publication Date (W3CDTF): 2017-03-24
• Degree grantor/type: 三重大学
• Date Granted: 2017-03-24
• Date Granted (W3CDTF): 2017-03-24
• Dissertation Number: 甲医学第1814号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1814号
• Text Language Code: eng
• Alias of Author: カワモト, エイジ; 川本, 英嗣
• Subject Heading: Integrin; Thrombomodulin; Inflammation; Adhesion molecules
• Target Audience: 一般
• Note (General): application/pdf; LFA-1 (aLb2) and Mac-1 (aMb2) integrins regulate leukocyte trafficking in health and disease by binding primarily to IgSF ligand ICAM-1 and ICAM-2 on endothelial cells. Here we have shown that the anticoagulant molecule thrombomodulin (TM), found on the surface of endothelial cells, functions as a potentially new ligand for leukocyte integrins. We generated a recombinant extracellular domain of human TM and Fc fusion protein (TM-domains 123-Fc), and showed that pheripheral blood mononuclear cells (PBMCs) bind to TM-domains 123-Fc dependent upon integrin activation. We then demonstrated that aL integrin-blocking mAb, aM integrin-blocking mAb, and b2 integrin-blocking mAb inhibited the binding of PBMCs to TM-domains 123-Fc. Furthermore, we show that the serine/threonine-rich domain (domain 3) of TM is required for the interaction with the LFA-1 (aLb2) and Mac-1 (aMb2) integrins to occur on PBMCs. These results demonstrate that the LFA-1 and Mac-1 integrins on leukocytes bind to TM, thereby establishing the molecular and structural basis underlying LFA-1 and Mac-1 integrin interaction with TM on endothelial cells. In fact, integrin-TM interactions might be involved in the dynamic regulation of leukocyte adhesion with endothelial cells.; 本文 / Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine; Emergency and Critical Care Center, Mie University Hospital; 8p
• DOI: info:doi/10.1016/j.bbrc.2016.04.007; https://doi.org/info:doi/10.1016/j.bbrc.2016.04.007
• Persistent ID (NDL): info:ndljp/pid/10993793; https://dl.ndl.go.jp/pid/10993793
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-12-04T02:02:48+09:00
• Date Created (W3CDTF): 2017-11-07
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10076/00017114; https://mie-u.repo.nii.ac.jp/records/11470
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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