An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas

• Material Type: 博士論文
• Title: An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas
• Author/Editor: Yamamichi, Akane
• Author Heading: Yamamichi, Akane
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2017-03-24
• Publication Date (W3CDTF): 2017-03-24
• Degree grantor/type: 三重大学
• Date Granted: 2017-03-24
• Date Granted (W3CDTF): 2017-03-24
• Dissertation Number: 甲医学第1829号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1829号
• Text Language Code: eng
• Alias of Author: ヤマミチ, アカネ; 山道, 茜
• Subject Heading: Glioma; isocitrate dehydrogenase 1 mutation; immuno-wall microdevice; rapid diagnosis; precision medicine
• Target Audience: 一般
• Note (General): application/pdf; World Health Organization grade II and III gliomas most frequently occur in the central nervous system (CNS) in adults. Gliomas are not circumscribed; tumor edges are irregular and consist of tumor cells, normal brain tissue, and hyperplastic reactive glial cells. Therefore, the tumors are not fully resectable, resulting in recurrence, malignant progression, and eventual death. Approximately 69–80% of grade II and III gliomas harbor mutations in the isocitrate dehydrogenase 1 gene (IDH1), of which 83–90% are found to be the IDH1-R132H mutation. Detection of the IDH1-R132H mutation should help in the differential diagnosis of grade II and III gliomas from other types of CNS tumors and help determine the boundary between the tumor and normal brain tissue. In this study, we established a highly sensitive antibody-based device, referred to as the immuno-wall, to detect the IDH1-R132H mutation in gliomas. The immuno-wall causes an immunoreaction in microchannels fabricated using a photo-polymerizing polymer. This microdevice enables the analysis of the IDH1 status with a small sample within 15 min with substantially high sensitivity. Our results suggested that 10% content of the IDH1-R132H mutation in a sample of 0.33 μl volume, with 500 ng protein, or from 500 cells is theoretically sufficient for the analysis. The immuno-wall device will enable the rapid and highly sensitive detection of the IDH1-R132H mutation in routine clinical practice.; 本文 / Department of Neurosurgery, Nagoya University Graduate School of Medicine; Department of Neurosurgery, Mie University Graduate School of Medicine; 9p
• DOI: info:doi/10.1080/14686996.2016.1227222; https://doi.org/info:doi/10.1080/14686996.2016.1227222
• Persistent ID (NDL): info:ndljp/pid/10993806; https://dl.ndl.go.jp/pid/10993806
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-12-04T02:02:48+09:00
• Date Created (W3CDTF): 2017-11-07
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10076/00017127; https://mie-u.repo.nii.ac.jp/records/11483
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-12-15 再収集