In vitro shoot grafting on rootstock : an effective tool for Agrobacterium-mediated transformation of pigeonpea (Cajanus cajan (L.) Millsp.)

• Material Type: 記事
• Title: In vitro shoot grafting on rootstock : an effective tool for Agrobacterium-mediated transformation of pigeonpea (Cajanus cajan (L.) Millsp.)
• Author/Editor: Gourab Ghosh; Arnab Purohit; Shreeparna Ganguly
• Publication, Distribution, etc.: 日本 : 日本植物細胞分子生物学会
• Publication Date: 2014
• Publication Date (W3CDTF): 2014
• Periodical title: Plant biotechnology
• No. or year of volume/issue: 31(4)
• Volume: 31(4)
• ISSN (Periodical Title): 1347-6114
• ISSN-L (Periodical Title): 1342-4580
• Text Language Code: eng
• DOI: 10.5511/plantbiotechnology.14.0805a; https://doi.org/10.5511/plantbiotechnology.14.0805a
• Persistent ID (NDL): info:ndljp/pid/11000376; https://dl.ndl.go.jp/pid/11000376
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会; https://dl.ndl.go.jp/collections/B00043
• Acquisition Basis: インターネット資料収集保存事業（WARP）
• Date Accepted (W3CDTF): 2017-12-08T10:56:38+09:00
• Date Captured (W3CDTF): 2015-08-15
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• Periodical Title (URI): https://warp.da.ndl.go.jp/info:ndljp/pid/9483390/www.jspcmb.jp/journal/pb31_4.html
• Periodical Title (Persistent ID (NDL)): info:ndljp/pid/11000374; https://dl.ndl.go.jp/pid/11000374
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Collection (particular): 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会; https://dl.ndl.go.jp/collections/B00043
• Access Restrictions: インターネット公開
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• Holding library: 国立国会図書館
• Call No.: Z54-J126
• Related Material (URI): http://dl.ndl.go.jp/info:ndljp/pid/11000376
• Related Material (Persistent ID (NDL)): info:ndljp/pid/11000376; http://dl.ndl.go.jp/info:ndljp/pid/11000376
• Data Provider (Database): 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• Bibliographic ID (NDL): 026003741; http://id.ndl.go.jp/bib/026003741
• Bibliographic Record Category (NDL): 632

• Summary, etc.: Lack of reproducible in vitro transformation method in pigeonpea limits the application of biotechnological breeding approaches for its genetic improvement. The present study describes a transformation method using novel in vitro shoot grafting technique for two cultivars ICPL87 and ICPL87119. Modified Murashige and Skoog (MS) medium with 1 mg l⁻¹ 6-benzylaminopurine and 0.2 mg l⁻¹ α-naphthaleneacetic acid induced an average of 25 shoots from decapitated embryonic axis explants after six weeks of culture. These shoots were further elongated in a modified MS medium containing 0.5 mg l⁻¹ 6-benzylaminopurine along with 0.5 mg l⁻¹ gibberellic acid for another four weeks. Grafting of pigeonpea shoots to seedling rootstock allowed 95% recovery of shoots. The whole regeneration process, starting from explant preparation to complete plant development, took 12–13 weeks. Further, the explants were infected with <i>Agrobacterium tumefaciens</i> harboring a binary vector pBI121. Transient and constitutive β-glucuronidase expressions were obtained in putative transgenic shoots selected at 100 mg l⁻¹ kanamycin. The selected shoots were grafted on non-transgenic root stock to establish putative transformants. T₀ and T₁ transformants were confirmed through polymerase chain reaction for presence of <i>neomycin phosphotransferase</i> gene. An overall 9% of transformation efficiency was recorded in both cultivars.
• DOI: 10.5511/plantbiotechnology.14.0805a
• Access Restrictions: インターネット公開
• Data Provider (Database): 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• Summary, etc.: Lack of reproducible in vitro transformation method in pigeonpea limits the application of biotechnological breeding approaches for its genetic improvement. The present study describes a transformation method using novel in vitro shoot grafting technique for two cultivars ICPL87 and ICPL87119. Modified Murashige and Skoog (MS) medium with 1 mg l⁻¹ 6-benzylaminopurine and 0.2 mg l⁻¹ α-naphthaleneacetic acid induced an average of 25 shoots from decapitated embryonic axis explants after six weeks of culture. These shoots were further elongated in a modified MS medium containing 0.5 mg l⁻¹ 6-benzylaminopurine along with 0.5 mg l⁻¹ gibberellic acid for another four weeks. Grafting of pigeonpea shoots to seedling rootstock allowed 95% recovery of shoots. The whole regeneration process, starting from explant preparation to complete plant development, took 12–13 weeks. Further, the explants were infected with <i>Agrobacterium tumefaciens</i> harboring a binary vector pBI121. Transient and constitutive β-glucuronidase expressions were obtained in putative transgenic shoots selected at 100 mg l⁻¹ kanamycin. The selected shoots were grafted on non-transgenic root stock to establish putative transformants. T₀ and T₁ transformants were confirmed through polymerase chain reaction for presence of <i>neomycin phosphotransferase</i> gene. An overall 9% of transformation efficiency was recorded in both cultivars.
• DOI: 10.5511/plantbiotechnology.14.0805a; https://doi.org/10.5511/plantbiotechnology.14.0805a
• Related Material (URI): http://id.ndl.go.jp/bib/026003741; https://ndlsearch.ndl.go.jp/books/R000000004-I026003741; https://dl.ndl.go.jp/pid/11000376; http://id.ndl.go.jp/digimeta/11000376
• References: A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures; https://cir.nii.ac.jp/crid/1360292617916423552; Somatic embryogenesis in cell suspension cultures of pigeonpea (Cajanus cajan); https://cir.nii.ac.jp/crid/1360292619094406912; Agrobacterium-mediated production of transgenic pigeonpea (Cajanus cajan L. Millsp.) expressing the synthetic BT cry1Ab gene; https://cir.nii.ac.jp/crid/1360855570668480128; Factors affecting regeneration of pigeonpea (Cajanus cajan L. Millsp) from mature embryonal axes; https://cir.nii.ac.jp/crid/1360855571265122560; Somatic Embryogenesis and Plant Regeneration in Pigeonpea; https://cir.nii.ac.jp/crid/1361137045846740992; High-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in <i>Arabidopsis</i>; https://cir.nii.ac.jp/crid/1361418519019606656; Differential Response of Cajanus cajan Varieties to Transformation with Different Strains of Agrobacterium; https://cir.nii.ac.jp/crid/1361418519432425856; Factors affecting high frequency differentiation of shoots and roots from cotyledon explants of Brassica juncea (L.) czern; https://cir.nii.ac.jp/crid/1361418519522242944; In vitro shoot-tip grafting improves recovery of cotton plants from culture; https://cir.nii.ac.jp/crid/1361418520887810944; Organogenesis and embryogenesis from diverse explants in pigeonpea (Cajanus cajan L.); https://cir.nii.ac.jp/crid/1361981471232704000; In vitro regeneration and transformation of pigeonpea [Cajanus cajan (L.) Millsp]; https://cir.nii.ac.jp/crid/1362544419039791232; Regeneration of pigeonpea (Cajanus cajan) from cotyledonary node via multiple shoot formation; https://cir.nii.ac.jp/crid/1362544420227996672; Plant regeneration via somatic embryogenesis in pigeonpea ( Cajanus cajan L. Millsp); https://cir.nii.ac.jp/crid/1362825893348967552; Expression of a synthetic <i>cry</i>1AcF gene in transgenic Pigeon pea confers resistance to <i>Helicoverpa armigera</i>; https://cir.nii.ac.jp/crid/1363107369892195200; Thidiazuron-induced shoot regeneration in pigeonpea (Cajanus cajan L.); https://cir.nii.ac.jp/crid/1363388843255374080; Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.); https://cir.nii.ac.jp/crid/1363388844798093824; Agrobacterium-mediated genetic transformation of pigeon pea (Cajanus cajan (L.) Millsp.) using embryonal segments and development of transgenic plants for resistance against Spodoptera; https://cir.nii.ac.jp/crid/1363388845749405824; An efficient protocol for shoot regeneration and genetic transformation of pigeonpea [Cajanus cajan (L.) Millsp.] using leaf explants; https://cir.nii.ac.jp/crid/1363670319365888000; Expression of biologically active Hemagglutinin-neuraminidase protein of Peste des petits ruminants virus in transgenic pigeonpea [Cajanus cajan (L) Millsp.]; https://cir.nii.ac.jp/crid/1363670319993275264; Nutrient requirements of suspension cultures of soybean root cells; https://cir.nii.ac.jp/crid/1363670320086928640; Genetic transformation of pigeonpea with rice chitinase gene; https://cir.nii.ac.jp/crid/1363670320561655552; Progress of tissue culture and genetic transformation research in pigeon pea [Cajanus cajan (L.) Millsp.]; https://cir.nii.ac.jp/crid/1363670320615080192; The effect of TDZ on organogenesis and somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp); https://cir.nii.ac.jp/crid/1363670320985930496; Plant regeneration from leaf discs of peanut and pigeonpea: Influence of benzyladenine, indoleacetic acid and indoleacetic acid-amino acid conjugates; https://cir.nii.ac.jp/crid/1364233269402487680; Plant Regeneration from Decapitated Mature Embryo Axis and Agrobacterium Mediated Genetic Transformation of Pigeonpea; https://cir.nii.ac.jp/crid/1364233269528300032; Efficient and rapid in vitro plant regeneration system for Indian cultivars of chickpea (Cicer arietinum L.); https://cir.nii.ac.jp/crid/1364233270286787200
• Data Provider (Database): 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• Original Data Provider (Database): Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• Bibliographic ID (NDL): 026003741; http://id.ndl.go.jp/bib/026003741; 11000376; http://id.ndl.go.jp/bib/11000376
• NAID: 130004690905; 40020313497