Flower color modification in Rosa hybrida by expressing the S-adenosylmethionine : anthocyanin 3′,5′-O-methyltransferase gene from Torenia hybrida
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DOI[10.5511/plantbiotechnology.15.0205a]to the data of the same series
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- Material Type
- 記事
- Author/Editor
- Noriko NakamuraYukihisa KatsumotoFilippa Brugliera
- Publication, Distribution, etc.
- Publication Date
- 2015
- Publication Date (W3CDTF)
- 2015
- Periodical title
- Plant biotechnology
- No. or year of volume/issue
- 32(2)
- Volume
- 32(2)
- ISSN (Periodical Title)
- 1347-6114
- ISSN-L (Periodical Title)
- 1342-4580
- Text Language Code
- eng
- DOI
- 10.5511/plantbiotechnology.15.0205a
- Persistent ID (NDL)
- info:ndljp/pid/11000425
- Collection
- Collection (Materials For Handicapped People:1)
- Collection (particular)
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会
- Acquisition Basis
- インターネット資料収集保存事業（WARP）
- Date Accepted (W3CDTF)
- 2017-12-08T10:56:38+09:00
- Date Captured (W3CDTF)
- 2015-08-15
- Format (IMT)
- application/pdf
- Access Restrictions
- インターネット公開
- Availability of remote photoduplication service
- 不可
- Periodical Title (URI)
- Periodical Title (Persistent ID (NDL))
- info:ndljp/pid/11000424
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館デジタルコレクション
- Collection (particular)
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会
- Access Restrictions
- インターネット公開
- Availability of remote photoduplication service
- 不可
- Holding library
- 国立国会図書館
- Call No.
- Z54-J126
- Related Material (URI)
- Related Material (Persistent ID (NDL))
- info:ndljp/pid/11000425
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館雑誌記事索引
- Bibliographic ID (NDL)
- 026560107
- Bibliographic Record Category (NDL)
- 632
- Summary, etc.
- We isolated a cDNA encoding <i>S</i>-adenosylmethionine: anthocyanin 3′,5′-<i>O</i>-methyltransferase (A3′5′OMT) from a cDNA library derived from <i>Torenia hybrida</i> petals that mainly accumulated malvidin type anthocyanins using the petunia A3′OMT cDNA as a probe. The torenia A3′5′OMT shared 52–72% amino acid sequence identity with previously reported AOMTs and belongs to the Group A1 methyltransferase family that also include caffeoyl CoA <i>O</i>-methyltransferase. The recombinant A3′5′OMT produced by <i>Escherichia coli</i> efficiently catalyzed methylation of the 3-glucoside and 3,5-diglucoside of delphinidin and cyanidin, but it did not catalyze the methylation of anthocyanidins, flavonols, or flavones. The torenia <i>A3</i>′<i>5</i>′<i>OMT</i> gene was expressed in <i>Nierembergia</i> sp., the petals of which naturally accumulate anthocyanins derived from delphinidin. The resultant transgenic petals produced methylated anthocyanins, based on malvidin and petunidin, in addition to delphinidin, which indicated that the torenia <i>A3</i>′<i>5</i>′<i>OMT</i> gene was functional in a heterologous plant. Rose petals rarely contain methylated anthocyanins. Transgenic rose petals expressing both a pansy <i>flavonoid 3′,5′-hydroxylase</i> (<i>F3</i>′<i>5</i>′<i>H</i>) and the torenia <i>A3</i>′<i>5</i>′<i>OMT</i> genes accumulated methylated anthocyanins based upon malvidin, petunidin, and peonidin, which comprised up to 88% of the total anthocyanidins, and their magenta color was more brilliant than that of the petals that accumulated delphinidin type anthocyanins by expressing the <i>F3</i>′<i>5</i>′<i>H</i> gene alone. These results indicate that the torenia <i>A3</i>′<i>5</i>′<i>OMT</i> gene is a useful molecular tool for altering and diversifying flower color.
- DOI
- 10.5511/plantbiotechnology.15.0205a
- Access Restrictions
- インターネット公開
- Data Provider (Database)
- 科学技術振興機構 : J-STAGE
- Summary, etc.
- We isolated a cDNA encoding <i>S</i>-adenosylmethionine: anthocyanin 3′,5′-<i>O</i>-methyltransferase (A3′5′OMT) from a cDNA library derived from <i>Torenia hybrida</i> petals that mainly accumulated malvidin type anthocyanins using the petunia A3′OMT cDNA as a probe. The torenia A3′5′OMT shared 52–72% amino acid sequence identity with previously reported AOMTs and belongs to the Group A1 methyltransferase family that also include caffeoyl CoA <i>O</i>-methyltransferase. The recombinant A3′5′OMT produced by <i>Escherichia coli</i> efficiently catalyzed methylation of the 3-glucoside and 3,5-diglucoside of delphinidin and cyanidin, but it did not catalyze the methylation of anthocyanidins, flavonols, or flavones. The torenia <i>A3</i>′<i>5</i>′<i>OMT</i> gene was expressed in <i>Nierembergia</i> sp., the petals of which naturally accumulate anthocyanins derived from delphinidin. The resultant transgenic petals produced methylated anthocyanins, based on malvidin and petunidin, in addition to delphinidin, which indicated that the torenia <i>A3</i>′<i>5</i>′<i>OMT</i> gene was functional in a heterologous plant. Rose petals rarely contain methylated anthocyanins. Transgenic rose petals expressing both a pansy <i>flavonoid 3′,5′-hydroxylase</i> (<i>F3</i>′<i>5</i>′<i>H</i>) and the torenia <i>A3</i>′<i>5</i>′<i>OMT</i> genes accumulated methylated anthocyanins based upon malvidin, petunidin, and peonidin, which comprised up to 88% of the total anthocyanidins, and their magenta color was more brilliant than that of the petals that accumulated delphinidin type anthocyanins by expressing the <i>F3</i>′<i>5</i>′<i>H</i> gene alone. These results indicate that the torenia <i>A3</i>′<i>5</i>′<i>OMT</i> gene is a useful molecular tool for altering and diversifying flower color.
- DOI
- 10.5511/plantbiotechnology.15.0205a
- Access Restrictions
- インターネット公開
- Related Material (URI)
- Is Referenced By
- Diversification of Chemical Structures of Methoxylated Flavonoids and Genes Encoding Flavonoid-O-MethyltransferasesMolecular cloning of flavonoid biosynthetic genes and biochemical characterization of anthocyanin O-methyltransferase of Nemophila menziesii Hook. and ArnRecent advances in the research and development of blue flowers
- References
- Cloning, Functional Identification and Sequence Analysis of Flavonoid 3′-hydroxylase and Flavonoid 3′,5′-hydroxylase cDNAs Reveals Independent Evolution of Flavonoid 3′,5′-hydroxylase in the Asteraceae FamilyBiochemical and Molecular Characterization of a Novel UDP-Glucose:Anthocyanin 3′-<i>O</i>-Glucosyltransferase, a Key Enzyme for Blue Anthocyanin Biosynthesis, from GentianCloning and molecular analysis of structural genes involved in anthocyanin biosynthesis and expressed in a forma-specific manner in Perilla frutescensA DNA Transformation–Competent Arabidopsis Genomic Library in AgrobacteriumMetabolite annotations based on the integration of mass spectral informationTapetum‐specific location of a cation‐dependent <i>O</i>‐methyltransferase in <i>Arabidopsis thaliana</i>Spectral methods of characterizing anthocyaninsCyclic malyl anthocyanins in Dianthus caryophyllusCharacterization of a Vitis vinifera cv. Cabernet Sauvignon 3′,5′-O-methyltransferase showing strong preference for anthocyanins and glycosylated flavonolsFlower colour and cytochromes P450 <sup/>A Novel Cation-Dependent<i>O-</i>Methyltransferase Involved in Anthocyanin Methylation in GrapevineAnthocyanins in flowers of genus Rosa, sections Cinnamomeae (=Rosa), Chinenses, Gallicanae and some modern garden rosesA Novel Mg2+-dependent O-Methyltransferase in the Phenylpropanoid Metabolism of Mesembryanthemum crystallinumMolecular and biochemical characterization of torenia flavonoid 3′-hydroxylase and flavone synthase II and modification of flower color by modulating the expression of these genesGenetic Control and Evolution of Anthocyanin MethylationTranscription factors, sucrose, and sucrose metabolic genes interact to regulate potato phenylpropanoid metabolismBlue flower color development by anthocyanins: from chemical structure to cell physiologyBiosynthesis of plant pigments: anthocyanins, betalains and carotenoidsStructure, function, and evolution of plant<i>O</i>-methyltransferasesMetabolic Engineering of Flower Color Pathways Using Cytochromes P450Isolation and characterization of the fragrant cyclamen O-methyltransferase involved in flower colorationFlower flavonol and anthocyanin distribution in subgenus RosaAn <i>O‐</i>methyltransferase modifies accumulation of methylated anthocyanins in seedlings of tomatoThe Evolution of Flavonoids and Their GenesEfficient Promoter Cassettes for Enhanced Expression of Foreign Genes in Dicotyledonous and Monocotyledonous PlantspBINPLUS: An improved plant transformation vector based on pBIN19Engineering of the Rose Flavonoid Biosynthetic Pathway Successfully Generated Blue-Hued Flowers Accumulating DelphinidinGeneration of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidinRed-purple flower due to delphinidin 3,5-diglucoside, a novel pigment for Cyclamen spp., generated by ion-beam irradiationIdentification of Cyanidin 3-<i>O</i>-(3″,6″-<i>O</i>-Dimalonyl-<i>β</i>-glucopyranoside) as a Flower Pigment of Chrysanthemum (<i>Dendranthema grandiflorum</i>)Molecular characterization of the flavonoid biosynthetic pathway and flower color modification of Nierembergia sp
- Data Provider (Database)
- 国立情報学研究所 : CiNii Research
- Original Data Provider (Database)
- Japan Link Center雑誌記事索引データベース雑誌記事索引データベースCrossrefCiNii ArticlesCrossrefCrossrefCrossref
- Bibliographic ID (NDL)
- 02656010711000425
- NAID
- 130005085989