Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.) and field performance of somatic embryo-derived trees
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DOI[10.5511/plantbiotechnology.15.0408a]to the data of the same series
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- Material Type
- 記事
- Author/Editor
- Yoshihisa HosoiNoritsugu KuramotoTsuyoshi E. Maruyama
- Publication, Distribution, etc.
- Publication Date
- 2015
- Publication Date (W3CDTF)
- 2015
- Periodical title
- Plant biotechnology
- No. or year of volume/issue
- 32(2)
- Volume
- 32(2)
- ISSN (Periodical Title)
- 1347-6114
- ISSN-L (Periodical Title)
- 1342-4580
- Text Language Code
- eng
- DOI
- 10.5511/plantbiotechnology.15.0408a
- Persistent ID (NDL)
- info:ndljp/pid/11000431
- Collection
- Collection (Materials For Handicapped People:1)
- Collection (particular)
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会
- Acquisition Basis
- インターネット資料収集保存事業（WARP）
- Date Accepted (W3CDTF)
- 2017-12-08T10:56:38+09:00
- Date Captured (W3CDTF)
- 2015-08-15
- Format (IMT)
- application/pdf
- Access Restrictions
- インターネット公開
- Availability of remote photoduplication service
- 不可
- Periodical Title (URI)
- Periodical Title (Persistent ID (NDL))
- info:ndljp/pid/11000424
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館デジタルコレクション
- Collection (particular)
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会
- Access Restrictions
- インターネット公開
- Availability of remote photoduplication service
- 不可
- Holding library
- 国立国会図書館
- Call No.
- Z54-J126
- Related Material (URI)
- Related Material (Persistent ID (NDL))
- info:ndljp/pid/11000431
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館雑誌記事索引
- Bibliographic ID (NDL)
- 026560183
- Bibliographic Record Category (NDL)
- 632
- Summary, etc.
- In this study, 100 random amplified polymorphic DNA (RAPD) primers were screened to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.). Of the 100 primers tested, eight were selected based on their amplification products, which showed clear DNA fragmentation, generating 8.75 bands per primer in average. The amplification products showed no genetic variation among the tested somatic embryos. In addition, the performance of somatic embryo-derived trees was monitored in the field, and their mean height growth was measured until the age of 10 years. Although no phenotypic differences were observed, the somatic embryo-derived trees showed lower growth compared with seedlings.
- DOI
- 10.5511/plantbiotechnology.15.0408a
- Access Restrictions
- インターネット公開
- Data Provider (Database)
- 科学技術振興機構 : J-STAGE
- Summary, etc.
- In this study, 100 random amplified polymorphic DNA (RAPD) primers were screened to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.). Of the 100 primers tested, eight were selected based on their amplification products, which showed clear DNA fragmentation, generating 8.75 bands per primer in average. The amplification products showed no genetic variation among the tested somatic embryos. In addition, the performance of somatic embryo-derived trees was monitored in the field, and their mean height growth was measured until the age of 10 years. Although no phenotypic differences were observed, the somatic embryo-derived trees showed lower growth compared with seedlings.
- DOI
- 10.5511/plantbiotechnology.15.0408a
- Access Restrictions
- インターネット公開
- Related Material (URI)
- Is Referenced By
- Progress in Somatic Embryogenesis of Japanese Pines
- References
- Cellular and Molecular Genetic Improvement of CerealsDetermination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markersDNA polymorphisms amplified by arbitrary primers are useful as genetic markersEvidence of Somaclonal Variation in Somatic Embryo-Derived Plantlets of White Spruce (Picea Glauca (Moench) VossDevelopmental and genetic variation in nuclear microsatellite stability during somatic embryogenesis in pineGenetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markersRandom Amplified Polymorphic DNA (RAPD) Analysis of Long-term Cultured Hybrid HazelnutInterior spruce seedlings compared with emblings produced from somatic embryogenesis. I. Nursery development, fall acclimation, and over-winter storageRandom amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides MarshRAPD analysis of somaclonal variants derived from embryo callus cultures of peachMicropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markersIn Vitro Culture of Higher PlantsSomaclonal Variation in Tissue Culture: A Case Study with OliveClonal Propagation: GymnospermsGenetic parameters and performance stability of white spruce somatic seedlings in clonal testsPlant regeneration through somatic embryogenesis and rapd analysis of regenerated plants in Tylophora indica (Burm. F. Merrill.)Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)Biochemical events during germination and early growth of somatic embryos and seed of interior spruce (<i>Picea glauca engelmanni</i>complex)PCR-based molecular markers for assessment of somaclonal variation in Pinus pinea clones micropropagated in vitroSOMACLONAL VARIATION: HAS IT PROVED USEFUL FOR PLANT IMPROVEMENT?Polyethylene glycol promotes maturation but inhibits further development of <i>Picea abies</i> somatic embryosPlant regeneration through somatic embryogenesis from tissues of mature oak trees: true-to-type conformity of plantlets by RAPD analysisSomatic Embryogenesis in Sawara Cypress (<i>Chamaecyparis pisifera</i>Sieb. et Zucc.) for Stable and Efficient Plant Regeneration, Propagation and Protoplast CultureApplication of somatic embryogenesis in high-value clonal forestry: Deployment, genetic control, and stability of cryopreserved clonesCritical Factors Affecting <i>Ex Vitro</i> Performance of Somatic Embryo Plants of <i>Picea abies</i>Occurrence of Somaclonal Variation Among Somatic Embryo-Derived White Spruces (Picea glauca, Pinaceae)The Physiology of in Vitro Asexual EmbryogenesisInterior spruce seedlings compared with emblings produced from somatic embryogenesis. III. Physiological response and morphological development on a reforestation siteInterior spruce seedlings compared with emblings produced from somatic embryogenesis. II. Stock quality assessment prior to field plantingSources and Frequency of Somaclonal VariationSomaclonal Variation: Mechanism and Applications in Crop ImprovementRAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P.Somaclonal variation as a tool for crop improvementSomatic Embryogenesis in Woody PlantsMonitoring Genetic stability in<i>Quercus serrata</i>Thunb. Somatic Embryogenesis Using RAPD MarkersSomatic embryogenesis and somaclonal variation in Norway spruce: morphogenetic, cytogenetic and molecular approachesComparison of growth and stem form characters of<i>Pinus sylvestris</i>clones and seedlings of the same origin in a 10-year field trialIn vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantletsMicropropagation of Tectona grandis: Assessment of Genetic FidelityInfluence of regeneration method and tissue source on the frequency of somatic variation in Populus to infection by Septoria musivaMicropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markersEarly selection improves clonal performance and reduces intraclonal variation of Norway spruce plants propagated by somatic embryogenesisOrigins, Causes and Uses of Variation in Plant Tissue CulturesEmbryogenic Cell Culture, Protoplast Regeneration, Cryopreservation, Biolistic Gene Transfer and Plant Regeneration in Japanese Cedar (Cryptomeria japonica D. Don).
- Data Provider (Database)
- 国立情報学研究所 : CiNii Research
- Original Data Provider (Database)
- Japan Link Center雑誌記事索引データベース雑誌記事索引データベースCrossrefCiNii ArticlesCrossref
- Bibliographic ID (NDL)
- 02656018311000431
- NAID
- 130005085994