LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity

• Material Type: 博士論文
• Title: LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity
• Author/Editor: Li, Yuan
• Author Heading: Li, Yuan
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2016-11-18
• Publication Date (W3CDTF): 2016-11-18
• Alternative Title: LUC7L3/CROPはエンハンサーII/ベーサルコアプロモーターを抑制することによりB型肝炎ウイルスの複製を阻害する
• Periodical title: Scientific Reports
• Degree grantor/type: 浜松医科大学
• Date Granted: 2017-02-09
• Date Granted (W3CDTF): 2017-02-09
• Dissertation Number: 甲第742号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第742号
• Text Language Code: eng
• Subject Heading: Hepatitis B virus; HBV; replication; promoter; enhancer
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; The core promoter of hepatitis B virus (HBV) genome is a critical region for transcriptional initiation of 3.5 kb, pregenome and precore RNAs and for the viral replication. Although a number of host-cell factors that potentially regulate the viral promoter activities have been identified, the molecular mechanisms of the viral gene expression, in particular, regulatory mechanisms of the transcriptional repression remain elusive. In this study, we identified LUC7 like 3 pre-mRNA splicing factor (LUC7L3, also known as hLuc7A or CROP) as a novel interacting partner of HBV enhancer II and basal core promoter (ENII/BCP), key elements within the core promoter, through the proteomic screening and found that LUC7L3 functions as a negative regulator of ENII/BCP. Gene silencing of LUC7L3 significantly increased expression of the viral genes and antigens as well as the activities of ENII/BCP and core promoter. In contrast, overexpression of LUC7L3 inhibited their activities and HBV replication. In addition, LUC7L3 possibly contributes to promotion of the splicing of 3.5 kb RNA, which may also be involved in negative regulation of the pregenome RNA level. This is the first to demonstrate the involvement of LUC7L3 in regulation of gene transcription and in viral replication.
• DOI: info:doi/10.1038/srep36741; https://doi.org/info:doi/10.1038/srep36741
• Persistent ID (NDL): info:ndljp/pid/11050643; https://dl.ndl.go.jp/pid/11050643
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-03-04T07:19:18+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/3215; https://hama-med.repo.nii.ac.jp/records/3074
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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