Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer’s disease

• Material Type: 博士論文
• Title: Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer’s disease
• Volume: 7
• Author/Editor: Vu, Thi Hang
• Author Heading: Vu, Thi Hang
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2017-01-09
• Publication Date (W3CDTF): 2017-01-09
• Alternative Title: カイニン酸誘発てんかん様発作およびアルツハイマー病の両方における海馬神経細胞の神経突起でのαチューブリン修飾の増加
• Periodical title: Scientific Reports
• Degree grantor/type: 浜松医科大学
• Date Granted: 2017-03-13
• Date Granted (W3CDTF): 2017-03-13
• Dissertation Number: 甲第752号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第752号
• Text Language Code: eng
• Subject Heading: Post-translational modification; glutamylation; Δ2-tubulin; tubulin tyrosine ligase-like; cytosolic carboxypeptidase; neurodegeneration
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Neurodegeneration includes acute changes and slow-developing alterations, both of which partly involve common cellular machinery. During neurodegeneration, neuronal processes are impaired along with dysregulated post-translational modifications (PTMs) of cytoskeletal proteins. In neuronal processes, tubulin undergoes unique PTMs including a branched form of modification called glutamylation and loss of the C-terminal tyrosine residue and the penultimate glutamic acid residue forming Δ2-tubulin. Here, we investigated the state of two PTMs, glutamylation and Δ2 form, in both acute and slow-developing neurodegenerations, using a newly generated monoclonal antibody, DTE41, which had 2-fold higher affinity to glutamylated Δ2-tubulin, than to unmodified Δ2-tubulin. DTE41 recognised glutamylated Δ2-tubulin preferentially in immunostaining than in enzyme-linked immunosorbent assay and immunoblotting. In normal mouse brain, DTE41 stained molecular layer of the cerebellum as well as synapse-rich regions in pyramidal neurons of the cerebral cortex. In kainic acid-induced epileptic seizure, DTE41-labelled signals were increased in the hippocampal CA3 region, especially in the stratum lucidum. In the hippocampi of post-mortem patients with Alzheimer’s disease, intensities of DTE41 staining were increased in mossy fibres in the CA3 region as well as in apical dendrites of the pyramidal neurons. Our findings indicate that glutamylation on Δ2-tubulin is increased in both acute and slow-developing neurodegeneration.
• DOI: info:doi/10.1038/srep40205; https://doi.org/info:doi/10.1038/srep40205
• Persistent ID (NDL): info:ndljp/pid/11063587; https://dl.ndl.go.jp/pid/11063587
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-04-03T03:53:09+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/3221; https://hama-med.repo.nii.ac.jp/records/3079
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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