Selective improvement of peptides imaging on tissue by supercritical fluid wash of lipids for matrix-assisted laser desorption/ionization mass spectrometry

• Material Type: 博士論文
• Title: Selective improvement of peptides imaging on tissue by supercritical fluid wash of lipids for matrix-assisted laser desorption/ionization mass spectrometry
• Volume: 409 6
• Author/Editor: Matsushita, Shoko
• Author Heading: Matsushita, Shoko
• Publication, Distribution, etc.: Springer Berlin Heidelberg
• Publication Date: 2016-12-09
• Publication Date (W3CDTF): 2016-12-09
• Alternative Title: 脂質の超臨界流体洗浄による組織中ペプチドのマトリックス支援レーザー脱離/イオン化イメージング質量分析の選択的改善
• Periodical title: Analytical and Bioanalytical Chemistry
• Degree grantor/type: 浜松医科大学
• Date Granted: 2017-03-13
• Date Granted (W3CDTF): 2017-03-13
• Dissertation Number: 甲第753号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第753号
• Text Language Code: eng
• Subject Heading: imaging mass spectrometry; supercritical fluid of CO2; peptides; pretreatment; matrix-assisted laser desorption/ionization
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; There is a high analytical demand for improving the detection sensitivity for various peptides in matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) because exhaustive distribution analyses of various peptides could help to reveal the function of peptides in vivo. To improve the sensitivity of peptide detection, we used supercritical fluid of CO2 (scCO2) as washing solvent for a pretreatment to remove lipids. We evaluated whether our wash method using scCO2 with an entrainer improved the detection of peptides and suppressed lipid detection in MALDI-IMS. Our analysis revealed that the signal intensities of peptides such as m/z 3339.8, 3530.9, 4233.3, 4936.7, and 4963.7 were increased in scCO2-washed samples. The greatest improvement in the signal-to-noise ratio (S/N) was found at m/z 4963.7, which was identified as thymosin β4, with the S/N reaching almost 190-fold higher than the control. Additionally, all of the improved signals were associated with the morphologic structure. Our method allows us to analyze the distribution of molecules, especially in the region of m/z 3000?5200. For these improvements, the polarity difference between scCO2 and the matrix solution used was considered as a key. A wider variety of molecules can be analyzed in the future due to this improvement of the detection sensitivity by optimizing the polarity of scCO2 with various entrainers.
• DOI: info:doi/10.1007/s00216-016-0119-3; https://doi.org/info:doi/10.1007/s00216-016-0119-3
• Persistent ID (NDL): info:ndljp/pid/11063588; https://dl.ndl.go.jp/pid/11063588
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-04-03T03:53:09+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/3222; https://hama-med.repo.nii.ac.jp/records/3080
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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