Extracellular and intracellular mechanisms of mechanotransduction in three-dimensionally embedded rat chondrocytes.

• Material Type: 博士論文
• ISSN: 1932-6203
• Title: Extracellular and intracellular mechanisms of mechanotransduction in three-dimensionally embedded rat chondrocytes.
• Volume: 9 12
• Author/Editor: SHIOJI, Suguru
• Author Heading: SHIOJI, Suguru
• Publication, Distribution, etc.: Public Library of Science
• Publication Date: 2014-12-05
• Publication Date (W3CDTF): 2014-12-05
• Periodical title: PLoS ONE
• Degree grantor/type: 滋賀医科大学
• Date Granted: 2017-09-13
• Date Granted (W3CDTF): 2017-09-13
• Dissertation Number: 乙第434号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第434号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): Purpose:Articular cartilage homeostasis involves modulation of chondrocyte matrix synthesis in response to mechanical stress (MS). We studied extracellular and intracellular mechanotransduction pathways mediating this response.; Methods:We first confirmed rapid up-regulation of the putative chondro-protective cytokine, interleukin (IL)-4, as an immediate response to MS. We then studied the role of IL-4 by investigating responses to exogenous IL-4 or a specific IL-4 inhibitor, combined with MS. Next we investigated the intracellular second messengers. Since chondrocyte phenotype alters according to the extracellular environment, we characterized the response to mechanotransduction in 3-dimensionally embedded chondrocytes.; Results:Expression of aggrecan and type II collagen was significantly up-regulated by exogenous IL-4 whereas MS-induced matrix synthesis was inhibited by an IL-4 blocker. Further, MS-induced matrix synthesis was completely blocked by a p38 MAPK inhibitor, while it was only partially blocked by inhibitors of other putative second messengers.; Conclusion:IL-4 mediates an extracellular pathway of mechanotransduction, perhaps via an autocrine/paracrine loop, while p38 mediates an intracellular pathway prevalent only in a 3-dimensional environment.; Thesis or Dissertation; 平成29年度; 10.1371/journal.pone.0114327
• DOI: info:doi/10.1371/journal.pone.0114327; https://doi.org/info:doi/10.1371/journal.pone.0114327
• Persistent ID (NDL): info:ndljp/pid/11092367; https://dl.ndl.go.jp/pid/11092367
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-05-04T12:13:37+09:00
• Date Created (W3CDTF): 2018-04-23
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10422/00012410; https://shiga-med.repo.nii.ac.jp/records/3272
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2019-09-06 再収集

2023-07-18 再収集

2023-10-11 再収集